摘要
人脑红蛋白 (neuroglobin ,NGB)是新发现的神经系统特异的携氧蛋白 ,然而其全长cDNA序列一直未见报道 .采用电子序列延伸技术和cDNA序列末端快速扩增技术 (rapidamplificationofcDNAends ,RACE)研究发现 ,人NGB全长cDNA序列为 190 9bp,5′非编码区为 375bp ,编码区 (45 6bp)可编码 15 1个氨基酸 ,3′非编码区为 10 78bp,其中含 2 7bp的 poly (A) (GenBank接受号 :AF4 2 2 797) .综合采用电子序列延伸技术与RACE技术是获得全长cDNA序列的有效方法 ,为后续的功能研究提供了重要基础 .
Human neuroglobin (NGB) is a newly discovered gene functioned specifically in the oxygen supply and oxygen consumption in the brain. However, the full length cDNA sequence of NGB was not reported yet. Using the in silicon sequence elongation technique, 5′ RACE and 3′ RACE technique, the full length cDNA sequence of human neuroglobin was successfully obtained (GenBank accession number AF422797). The cDNA sequence of human NGB is 1 909 bp in size and capable of encoding a protein of 151 amino acids. The 5′ untranslated region is 375 bp, while the 3′ untranslated region is 1 078 bp including 27 bp of poly(A) sequence. Results demonstrated that the RACE technique coupled with electronic sequence elongation technique is useful to obtain unknown sequence of 5′ terminal or 3′ terminal part of a new gene.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2002年第6期946-951,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目 (3 0 10 0 0 493 990 0 0 413 990 0 0 74)
军事医学科学院科技创新研究启动基金 (0 10 2 0 0 1990 5 10 5 )部分资助项目~~
