摘要
目的 了解原发性肝细胞癌及其癌旁组织中P16、P5 3蛋白、上皮钙粘蛋白 (E cadherin)的表达。方法 用免疫组化方法检测 35例人肝细胞癌及其癌旁组织中P16、P5 3蛋白、上皮钙粘蛋白 (E cadherin)表达的改变 ,并结合临床资料进行分析。结果 肝癌和癌旁组织中P16、P5 3蛋白、上皮钙粘蛋白 (E cadherin)阳性率分别为 37.14 %、4 5 .71%、31.4 3%和 10 0 %、11.4 3%、71.4 3%。肝癌的P16蛋白、上皮钙粘蛋白 (E cadherin)表达率明显低于癌旁组织的表达率 ,P <0 .0 5 ,而P5 3蛋白的表达率明显高于癌旁组织的表达率 ,P <0 .0 5 ;P16蛋白、上皮钙粘蛋白 (E cadherin)阳性表达率在无转移的肝癌中分别为 82 .14 %、15 .38%。有转移组分别为 2 8.5 7%、4 0 .91% ;转移与非转移组比较有极显著性差异P <0 .0 1。P16、P5 3蛋白表达与组织学分级有明显关系P <0 .0 5。结论 P16蛋白缺失和 p5 3基因突变 ,与肝细胞癌增殖、分化程度及预后有关。E cadherin的丧失或低表达是肝癌获得高侵袭转移能力的因素之一。
Objective To comprehend the expressions of P16?P53 protein and E cadherin in tumor tissues , peritumor tissues of primary hepatocellular carcinoma Methods P16?P53 protein and E cadherin expressions were studied in 35 cases of HCC by using immunohistochemical method and compared with clinical data.Results P16?P53 protein and E cadherin positive expressions in the tumors and their neighboring regions were 37.14%?45.71%?31.43% and 100%? 11.43%?71.43% respectively?The positive expressions of P16 protein and E cadherin in HCC were significantly lower than those in their neighboring regions( P <0.05).The positive expressions of P53 protein in HCC were significantly higher than those in their neighboring regions( P <0.05).The positive expressions of P16 protein and E cadherin were respectively 82.14%?15.38% without metastasis in the carcinoma and 28.57% ?40.91% with metastasis, with an obvious difference between groups with and without metastasis( P <0.01).Expressions of P16?P53 protein was related to the histological grade.Conclusions P16 protein deletion and P53 gene mutation are closely related to proliferation,differentiation degree and prognosis of HCC.Loss or decreased expression of E cadherin was one of the factors for development of its highly invasive potentials.
出处
《重庆医学》
CAS
CSCD
2002年第12期1161-1163,共3页
Chongqing medicine
