摘要
目的探讨复方药王茶中总黄酮的最佳提取工艺。方法以总黄酮的含量为指标,通过单因素试验和正交实验相结合,以水为溶媒采用回流提取法,应用L 9(34)正交试验考察药材配伍比例、料液比、提取时间和提取次数对总黄酮提取率的影响。结果影响复方药王茶中总黄酮提取条件的因素主次顺序为,D提取次数>A药材配比>B料液比>C提取时间,优化的最佳提取工艺为药王茶与薄荷配比2∶1,料液比1∶8,回流提取3次,每次60 min。结论通过正交设计试验优选得到的复方药王茶中总黄酮提取工艺条件稳定、可靠,简单、总黄酮得率高、方法可行,为复方药王茶的开发应用提供了实验依据。
Objective To explore the optimal extraction process of total flavonoids in Compound Herb King Tea.Methods Using the content of total flavonoids as the indicator,a combination of single factor experiments and orthogonal experiments were conducted.Water was used as the solvent for reflux extraction,and L9(34)orthogonal experiment was applied to investigate the effects of medicinal material compatibility ratio,solid-liquid ratio,extraction time,and extraction times on the extraction rate of total flavonoids.Results The main and secondary factors affecting the extraction conditions of total flavonoids in Compound Herb King Tea are as follows:D extraction times>A medicinal herb ratio>B solid-liquid ratio>C extraction time.The optimized optimal extraction process is a 2∶1 ratio of Compound Herb King Tea to peppermint,a solid-liquid ratio of 1∶8,and reflux extraction for 3 times,each time for 60 minutes.Conclusion The optimal extraction process conditions for total flavonoids in Compound Herb King Tea obtained through orthogonal design experiment are stable,reliable,simple,with high total flavonoid yield and feasible method,providing experimental basis for the development and application of Compound Herb King Tea.
作者
董泽阳
王洋
张圻玮
张震
王小平
许建强
白吉庆
DONG Zeyang;WANG Yang;ZHANG Qiwei;ZHANG Zhen;WANG Xiaoping;XU Jianqiang;BAI Jiqing(Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China;Shaanxi Provincial Technical Service Center for Quality Monitoring of Traditional Chinese Medicine Raw Materials,Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China;Shaanxi Taibai Mountain Natural Plant Development Co.,Ltd.,Shaanxi Baoji 721600,China)
出处
《陕西中医药大学学报》
2025年第1期107-111,共5页
Journal of Shaanxi University of Chinese Medicine
基金
医疗服务与保障能力提升补助资金项目2019年中药资源普查专项(财社[2019]39号)
2021年陕西省大学生创新训练项目(S202110716078)。
关键词
复方药王茶
总黄酮
回流提取
单因素实验
正交试验
Compound Herb King Tea
Total flavonoids
Reflux extraction
Single factor experiment
Orthogonal test
