摘要
目的优化调肝活血稳压颗粒的提取工艺,并对其质量标准进行研究。方法本研究以浸膏得率及丹酚酸B的转移率的综合评分为指标,采用单因素考察结合Box-Behnken设计-响应面法优化调肝活血稳压颗粒的提取工艺,采用薄层色谱法对方中药材进行鉴别。结果确定最终提取工艺为:第一次加入10倍量水煎煮90 min,第二次加入6倍量水煎煮60 min。测定方法结果准确可靠。薄层色谱法检测出天麻、栀子、丹参等三味中药的特征性斑点。结论优化后的提取工艺操作简单,提取效果良好,结果稳定可靠。
Objective To optimize the extraction process of Tiaogan Huoxue Wenya granules and study its quality standards.Methods In this study,the comprehensive scores of extract yield and transfer rate of salvianolic acid B were used as indicators.Single factor investigation was combined with Box-Behnken design-response surface methodology to optimize the extraction process of Tiaogan Huoxue Wenya granules.Thin layer chromatography was used to identify the Chinese herbal medicine.Results The final extraction process was determined as follows:adding 10 times the amount of water and boiling for 90 minutes for the first time,and adding 6 times the amount of water and boiling for 60 minutes for the second time.The measurement method yields accurate and reliable results.Thin layer chromatography was used to detect characteristic spots in three traditional Chinese medicines:Tianma,Zhizi,and Danshen.Conclusion The optimized extraction process has simple operation,good extraction effect,and stable and reliable results.
作者
李凡
张国松
魏鑫华
贾超
夏明艳
李东勲
张文锍
LI Fan;ZHANG Guosong;WEI Xinhua;JIA Chao;XIA Mingyan;LI Donggui;ZHANG Wenxun(Jiangxi University of Chinese Medicine,Nanchang 330006,China;Jiangxi Provincial Hospital of Integrated Chinese and Western Medicine,Nanchang 330003,China;National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine,Nanchang 330006,China)
出处
《陕西中医药大学学报》
2025年第1期100-106,共7页
Journal of Shaanxi University of Chinese Medicine
基金
江西省教育厅科学技术研究项目(201234)
江西省中医药管理局科技计划项目(SZYYB20204497)。
关键词
调肝活血稳压颗粒
丹酚酸B
薄层色谱法
高效液相色谱法
Box-Behnken设计-响应面法
Tiaogan Huoxue Wenya granules
Danshensu acid B
Thin layer chromatography
High performance liquid chromatography method
Box-Behnken design-response surface methodology
