摘要
目的:优选栀子提取工艺。方法:以栀子苷为指标,用高效液相色谱仪,采用正交设计试验,考虑提取溶剂浓度、浸泡时间、提取时间、药材粒径等四种影响因素,对栀子提取工艺条件进行优选研究。结果:优选出的提取工艺条件为:栀子粗粉(20目)用80%甲醇溶液(8倍量)浸泡0.5h,超声提取2h,栀子苷的含量较高且稳定。结论:优选出的工艺经济、合理、稳定、可行,值得推广。
Objective：To optimize the extracting process of Gardenia.Methods：Using high performance liquid chromatography,with Geniposide as the index,the following four factors were considered：concentration of extraction solvent,soaking time,extracting time,and medicine particle size,the extracting process of Geniposide was optmized by the orthogonal design.Results：The optimization conditions of the extracting process was coarse powder of Gardenia（20 sieve）,80% methanol solution（eight times） as solvent,soaking time was half an hour and extraction time was two hours,the extraction of Geniposide was higher and stable.Conclusion：The optimization of the extraction process is economic,reasonable,stable and feasible.It can be widely used for the quality control of Fructus Gardeniae.
出处
《泸州医学院学报》
2011年第1期36-38,共3页
Journal of Luzhou Medical College
基金
四川省科技支撑计划项目(No.2010SZ0049)
四川省教育厅重点科研项目(No.2005A071)联合资助
关键词
栀子苷
提取工艺
正交设计试验
Geniposide
Extracting process
Orthogonal design
