摘要
为了明确裸花紫珠中的主要化学成分,研究不同干燥方式对裸花紫珠质量的影响,确定裸花紫珠适宜的产地加工方法,该研究利用UPLC-UV-Q-TOF-MS技术表征并鉴定了裸花紫珠中主要的苯乙醇苷类、黄酮类、环烯醚萜类等化合物35个,并选择其中紫外吸收较强、含量较高的8个化合物,建立了其同时含量测定的方法,用以评价不同加工方法裸花紫珠的质量。通过UPLC-UV-Q-TOF-MS结合PCA整体比较了阴干、晒干、不同温度(50、60、70、80、90、100℃)烘干及不同功率(119、231、385 W)微波干燥裸花紫珠样品的异同,进一步通过去咖啡酰基类叶升麻苷、胡黄连苷Ⅲ、木犀草苷、连翘酯苷B、毛蕊花糖苷、异毛蕊花糖苷、6-羟基木犀草苷、咖啡酸的含量变化,发现化合物总含量以及多数化合物的含量均呈现随着烘干温度升高而降低,随着微波功率的降低而降低的趋势。综合考虑样品中化合物含量、低碳、节能等需求,裸花紫珠生产过程中建议采用231 W微波干燥、低温烘干或自然晒干的干燥方式。该研究为裸花紫珠药材的产地干燥加工方法的选择及质量标准的提升提供了科学依据。
To identify the main chemical compounds of Callicarpae nudiflorae folium,investigate the impacts of different drying methods on its quality,and determine the appropriate processing method for Callicarpae nudiflora,this study employed UPLC-UV-Q-TOF-MS technology to characterize and identify 35 main compounds,including phenylethanoid glycosides,flavonoids,and iridoid compounds.Among these,a method for the simultaneous d etermination of 8 compounds with strong UV absorption and high content was established to evaluate the quality of Callicarpae nudiflora processed by different methods.By comparing the contents of verbasoside,picrosideⅢ,forsythin B,acteoside and isoact eoside in Callicarpa nudiflorae dried by microwave drying at different powers(119,231,and 385 w),drying-in-the-shade,sun drying and drying at different temperatures(50,60,70,80,90,and 100℃).It was discovered that the total content of compounds,as well as the content of most compounds,tends to decrease with the increase of drying temperature and with the decrease of microwave power.Taking into account the content of compounds in the sample,low carbon,energy saving and other requirements,it is recommended to use microwave drying at 231 w,low-temperature drying,or natural drying methods during the production process of Callicarpa nudiflora.This study provides a scientific basis for the selection of drying and processing methods for Callicarpa nudiflora at the place of origin and for the improvement of quality standards.
作者
侯晓燕
兰晓燕
朱利婉
邱子栋
李翔
周利
詹志来
黄胜
康利平
HOU Xiao-yan;LAN Xiao-yan;ZHU Li-wan;QIU Zi-dong;LI Xiang;ZHOU Li;ZHAN Zhilai;HUANG Sheng;KANG Li-ping(State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Hainan Jiuzhitang Pharmaceutical Co.,Ltd.,Haikou 570311,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第23期6320-6330,共11页
China Journal of Chinese Materia Medica
基金
中国中医科学院科技创新工程项目(CI2023E002,CI2023C071YLL)
中央本级重大增减支项目(2060302-2205-03)。
