摘要
为了探索不同产地两面针种子形态和发芽率的差异,本研究测定10个产地99份两面针种子的千粒重、表面积、周长、长、宽、长/宽和发芽率,计算变异系数和遗传多样性指数,进行主成分分析和聚类分析。结果表明,两面针种子形态和发芽性状存在丰富的遗传变异,其中变异系数最大的3个指标分别是15℃恒温发芽率(275.11%)、30℃/20℃昼夜变温发芽率(221.43%)、种子千粒重(16.00%)。种子的发芽率与种子千粒重不相关,与种子长/宽显著相关。主成分分析得出第1主成分反映种子的形态,第2主成分反映种子的发芽能力,第1主成分和第2主成分累积贡献率达82.47%。聚类分析将99份材料分为三类,类群Ⅰ种子千粒重大,类群Ⅱ发芽率高,类群Ⅲ种子千粒重小且发芽率低。筛选出发芽率高的种质3份,编号分别为JN2、MS5和YF8,可作为两面针种质资源的创新利用和新品种选育的亲本材料。
In order to explore the differences of seed morphology and germination rate of Zanthoxylum nitidum(Roxb.DC.)from different provenances,the 1000-grain weight,area,girth,length,width,length/width and germination rate of 99 seeds from 10 producing areas were measured,coefficient of variation and genetic diversity index were calculated in this study,and principal component analysis and cluster analysis were performed.The results showed that there were abundant genetic variation in seed morphology and germination traits,among which the three indexes with the largest coefficient of variation were the germination rate at 15℃(275.11%),the germination rate at 30℃/20℃diurnal temperature(221.43%),and the seed 1000-grain weight(16.00%).The germination rate of seed was not related to the 1000-grain weight of seed,but was significantly related to the length/width of seed.Principal component analysis showed that the first principal component reflected seed morphology,the second principal component reflected seed germination ability,and the cumulative contribution rate of the first and second principal components was 82.47%.The 99 materials were divided into three categories by cluster analysis.GroupⅠhad large 1000-grain weight and high germination rate,GroupⅡhad small 1000-grain weight and low germination rate.Three germplasm with high germination rate were selected,which were numbered JN2,MS5 and YF8 respectively,and could be used as parent materials for innovative utilization of germplasm resources and breeding of new varieties.
作者
董青松
张洪胜
李浩通
胡营
韦筱媚
黄宝优
朱艳霞
DONG Qingsong;ZHANG Hongsheng;LI Haotong;HU Ying;WEI Xiaomei;HUANG Baoyou;ZHU Yanxia(Medicinal Germplasm Resources Center,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;China Resources Sanjiu Medicinal&Pharmaceutical Co.,Ltd.,Shenzhen Guangdong 518109,China;Guangxi Guanglin Zhengshan Pharmaceutical Co.,Ltd.,Nanning 530022,China)
出处
《种子》
北大核心
2024年第10期85-92,共8页
Seed
基金
广西科技计划项目(桂科AB22035068)
广西药用资源保护与遗传改良重点实验室自主课题(KL2023ZZ03)
广西中医药管理局广西特色药材关键技术研究推广项目(GZKJ2314-5)。
关键词
两面针
种子形态
发芽率
遗传多样性分析
Zanthoxylum nitidum(Roxb.)DC.
seed morphology
germination rate
genetic diversity analysis
