摘要
目的 探讨非小细胞肺癌(non-small cell lung cancer, NSCLC)患者癌组织卷曲蛋白3(frizzled 3,FZD3)、蓬乱蛋白2(dishevelled 2,DVL2)表达水平及其临床预后意义。方法 选取2018-09/2020-09月作者医院收治的105例NSCLC患者为研究对象,手术留取癌组织和癌旁组织并制作成标本。采用免疫组织化学法检测FZD3、DVL2在癌组织和癌旁组织标本中的表达情况;分析FZD3、DVL2表达水平与NSCLC患者临床特征的关系,并分析影响NSCLC患者预后的相关因素。结果 癌组织中FZD3阳性表达率、DVL2阳性表达率均高于癌旁组织(P均<0.05)。临床分期为Ⅲ期、分化程度为低分化、有淋巴结转移的NSCLC患者FZD3阳性表达率和DVL2阳性表达率高于临床分期为Ⅱ期、分化程度为中/高分化、无淋巴结转移的NSCLC患者(P均<0.05)。FZD3阳性表达、DVL2阳性表达的NSCLC患者的3年总生存率低于FZD3阴性表达、DVL2阴性表达的NSCLC患者(P均<0.05)。多因素Cox回归分析显示,分化程度低(HR=2.524,95%CI:1.669~3.817)、有淋巴结转移(HR=2.959,95%CI:1.897~4.618)、FZD3阳性表达(HR=3.111,95%CI:1.982~4.883)、DVL2阳性表达(HR=3.476,95%CI:2.155~5.608)是NSCLC患者预后的独立危险因素(P<0.05)。结论 FZD3、DVL在NSCLC组织中表达上调,且与临床分期、分化程度、淋巴结转移有关,有望作为NSCLC预后评估的生物学标志物。
Objective To explore the expression levels and clinical prognostic significance of frizzled 3(FZD3)and dishevelled 2(DVL2)in cancer tissues of patients with non-small cell lung cancer(NSCLC).Methods A total of 105 pa-tients with NSCLC admitted to author's hospital from September 2018 to September 2020 were selected,cancer tissues and adjacent tissues were collected and made into specimens.The expression levels of FZD3 and DVL2 in the cancer tis-sues and adjacent tissues specimens were detected by immunohistochemistry;the relationship between the expression lev-els of FZD3,DVL2 and the clinical characteristics of NSCLC patients was analyzed,and the related factors affecting the prognosis of NSCLC patients were analyzed.Results The positive expression rates of FZD3 and DVL2 in the cancer tis-sues were higher than those in the adiacent tissues(all P<0.05).The positive expression rates of FZD3 and DVL2 in NSCLC patients with clinical stage II,poor differentiation and lymph node metastasis were higher than those in NSCLC patients with clinical stage II,medium/high differentiation and no lymph node metastasis(all P<0.05).The 3-year overall survival of NSCLC patients with FZD3 positive expression and DVL2 positive expression was lower than that of cases with FZD3 negative expression and DVL2 negative expression(all P<0.05).Multivariate Cox regression analysis showed that poor differentiation(HR=2.524,95%CI:1.669-3.817),lymph node metastasis(HR=2.959,95%CI:1.897-4.618),FZD3 positive expression(HR=3.111,95%CI:1.982-4.883)and DVL2 positive expression(HR=3.476,95%CI:2.155-5.608)were independent risk factors for the prognosis of NSCLC patients(P<0.05).Conclu-sion The expressions of FZD3 and DVL are up-regulated in NSCLC tissues and are related to clinical stage,differentia-tion degree and lymph node metastasis,which are expected to be used as biological markers for prognosis evaluation of NSCLC.
作者
段智慧
DUAN Zhihui(Department of Respiratory and Critical Care Medicine,Affiliated Hospital of Inner Mongolia Medi-cal University,Hohhot Inner Mongolia 010030,China)
出处
《联勤军事医学》
CAS
2024年第6期493-496,515,共5页
Military Medicine of Joint Logistics
基金
内蒙古医科大学科技百万工程联合项目[YKD2020KJBW(LH)041]。
