摘要
目的研究樟叶黄酮类成分及其抗氧化活性。方法通过液液萃取、大孔吸附树脂、HW-40C凝胶色谱、分子排阻色谱和制备型HPLC分离纯化樟叶95%乙醇提取物,根据理化性质及波谱数据鉴定化合物的结构。采用DPPH法测定其抗氧化活性。结果从中分离得到10个黄酮类化合物,分别鉴定为(2 R,3S)-7-methoxy-5-O-β-D-glucopyranosyl-afzelechin(1)、槲皮素-3-O-桑布双糖苷(2)、槲皮素-3-O-β-D-芹菜糖-(1→2)-β-D-葡萄糖苷(3)、槲皮素-3-O-洋槐糖苷(4)、山柰酚-3-O-β-D-芸香糖-7-O-β-D-葡萄糖苷(5)、山柰酚-3-O-α-L-鼠李糖-7-O-β-D-葡萄糖苷(6)、5,3′-di-O-methyl-(-)-epicatechin(7)、cinchonainⅡb(8)、槲皮素-3,4′-二-O-β-D-葡萄糖苷(9)、表儿茶素(10)。化合物8清除DPPH自由基的IC 50值为4.8μg/mL。结论化合物1为新化合物,化合物2~6为首次从樟属植物中分离鉴定,化合物7~9为首次从樟叶中分离得到。化合物8具有良好的抗氧化活性。
AIM To study the flavonoids from the leaves of Cinnamomum camphora(L.)Presl.and their antioxidant activities.METHODS The 95%ethanol extraction from the leaves of C.camphora was isolated and purified by liquid-liquid extraction,macroporous adsorption resin chromatography,HW-40C gel column chromatography,molecular exclusion chromatography and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antioxidant activity was determined by DPPH method.RESULT Ten flavonoids were isolated and identified as(2 R,3S)-7-methoxy-5-O-β-D-glucopyranosyl-afzelechin(1),quercetin-3-O-sambubioside(2),quercetin-3-O-β-D-apiosyl-(1→2)-β-D-glucoside(3),quercetin-3-O-robibioside(4),kaempferol-3-O-β-D-rutinoside-7-O-β-D-glucoside(5),kaempferol-3-O-α-L-rhamnoside-7-O-β-D-glucoside(6),5,3′-di-O-methyl-epicatechin(7)、cinchonainⅡb(8)、quercetin-3,4′-di-O-β-D-glucoside(9)、(-)-epicatechin(10).The IC 50 value of compound 8 scavenging DPPH free radical was 4.8μg/mL.CONCLUSION Compound 1 is a new compound,and compound 2-6 are obtained from Cinnamomum genus for the first time,compound 7-9 are first isolated from this plant.Compound 8 shows good antioxidant activities..
作者
杨鹏飞
卫金洪
钱玉梅
孙正光
吴薇
黄申
费嘉翔
毛多斌
YANG Peng-fei;WEI Jin-hong;QIAN Yü-mei;SUN Zheng-guang;WU Wei;HUANG Shen;FEI Jia-xiang;MAO Duo-bin(College of Tobacco Science and Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;Technology Center,Guangxi Tobacco Industrial Co.,Ltd.,Nanning 530000,China;Technology Center,Shenzhen Tobacco Industrial Co.,Ltd.,Shenzhen 518110,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第6期1889-1894,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(81903507)。
关键词
樟
叶
黄酮
分离鉴定
抗氧化活性
Cinnamomum camphora(L.)Presl
leaves
flavonoids
isolation and purification
antioxidant activities
