摘要
以3~5年生红花石蒜鳞茎为实验材料,研究不同激素处理和有机添加物对外植体诱导、增殖培养、生根培养的影响,筛选出红花石蒜高效组培再生体系。结果表明:采用双鳞片法,外植体进瓶诱导培养以MS+6-BA 5 mg·L^(-1)+IBA 3 mg·L^(-1)培养基为宜,平均分化芽数为2.53个;愈伤组织进瓶诱导以MS+TDZ 10 mg·L^(-1)+NAA 1 mg·L^(-1)为宜,愈伤组织诱导率达69.05%。芽的增殖培养基为MS+6-BA 5 mg·L^(-1)+IBA 3 mg·L^(-1)+椰汁50 g·L^(-1)最佳,平均分化芽数为4.13个,芽数量多,生长健壮;愈伤组织分化不定芽培养基以MS+TDZ 20 mg·L^(-1)+NAA 1 mg·L^(-1)最好,平均分化芽数为12.83个。最适生根培养基为MS+IBA 1 mg·L^(-1),生根率达100%。通过小鳞茎诱导芽的直接再生和愈伤组织诱导芽的间接再生两种方式获得了红花石蒜离体再生体系,为红花石蒜良种快繁和遗传转化体系构建奠定技术基础。
It was used 3-5 year old Lycoris radiata bulbs as experimental materials,an efficient tissue culture regeneration system was selected for L.radiata,through studying the effects of different hormone treatments and organic additives on explant induction,proliferation culture,and rooting culture.The results showed that using double-scale method,MS+6-BA 5 mg·L^(-1)+IBA 3 mg·L^(-1) medium was suitable for explant induction,the average number of differentiated buds was 2.53,and MS+TDZ 10 mg·L^(-1)+NAA 1 mg·L^(-1) was suitable for callus induction,and the callus induction rate was 69.05%.MS+6-BA 5 mg·L^(-1)+IBA 3 mg·L^(-1)+coconut juice 50 g·L^(-1) was the best medium for bud proliferation,the average number of differentiated buds was 4.13,the number of buds was large and the growth was robust,while MS+TDZ 20 mg·L^(-1)+NAA 1 mg·L^(-1) was the best medium for callus differentiation,and the average number of differentiated buds was 12.83.The optimum rooting medium was MS+IBA 1 mg·L^(-1),and the rooting rate was 100%.In vitro regeneration systems of L.radiata were obtained through two methods:direct regeneration of bulb-induced buds and indirect regeneration of callus-induced buds,which laid a technical foundation for rapid propagation and genetic transformation system of L.radiata.
作者
钟良涛
饶出林
龚岚
蔡军火
程强强
Zhong Liangtao;Rao Chulin;Gong Lan;Cai Junhuo;Cheng Qiangqiang(Colledge of Landscape Architecture and Art,Jiangxi Agricultural University,Nanchang Jiangxi 330045,China;Jiangxi Academy of Forestry,Modern Plant Tissue Culture Breeding Center,Nanchang Jiangxi 330013,China;Resource Protection and Development Center of Nanfeng County Forestry Bureau,Fuzhou Jiangxi 344599,China)
出处
《南方林业科学》
2023年第6期1-5,50,共6页
South China Forestry Science
基金
国家自然科学基金项目(项目编号:31960327)
江西省林业科学院基础研究与人才科研专项项目(项目编号:2023522001)。
关键词
红花石蒜
离体再生
不定芽
生根
Lycoris radiata
in vitro regeneration
adventitious buds
rooting
