摘要
目的利用GEO芯片联合网络药理学探讨青风藤治疗特发性膜性肾病(idiopathic membranous nephropathy,IMN)的可能作用机制。方法通过TCMSP数据库获取青风藤的活性成分和有效靶点,利用GEO芯片提取IMN的差异表达基因。借助Venny 2.1.0获取药物与疾病的共同靶点,采用Cytoscape 3.9.0构建“活性成分-靶点”网络。使用STRING数据库构建核心靶蛋白互作网络图,通过DAVID平台对有效靶点进行GO富集和KEGG通路富集分析,对主要成分与核心靶点进行分子对接验证并进行可视化分析。结果筛选获取青风藤有效活性成分6个,作用靶点52个,获得IMN差异表达基因3983个,药物与疾病交集基因8个。富集分析显示,青风藤可以影响对脂多糖的反应、凋亡信号通路等生物学过程,通过调控MAPK和cAMP等凋亡相关通路发挥治疗IMN作用。分子对接结果显示青风藤主要活性成分β-谷甾醇、拉兹马宁碱等与核心靶点JUN、CASP3等均能实现自发结合。结论青风藤可能通过作用于JUN和CASP3等关键靶点调控MAPK等信号通路,从而发挥调节细胞调亡、抑制炎症因子和调节免疫功能等作用来治疗IMN。
Objective To examine the possible mechanism of action of Qingfengteng(Sinomenii Caulis)in treating idiopathic membranous nephropathy(IMN)through GEO chip and network pharmacology.Methods The active ingredients and targets of Sinomenii Caulis were analysed using the TCMSP platform.Furthermore,deferentially expressed genes of IMN were extracted using the GEO chip.By utilising Venny 2.1.0,we identified the common targets of drugs and diseases.Subsequently,the“active ingredient-target”network was constructed through the application of Cytoscape 3.9.0.The core target protein interactions network map was created using the STRING database.The DAVID platform was utilized to carry out GO enrichment and KEGG pathway enrichment analyses of the effective targets.The molecular docking between the core targets and the main components was validated and visualized.Results A total of six active ingredients and 52 potential targets were screened in this study.The GEO data collected yielded 3983 differentially expressed genes in relation to IMN,along with eight drug-disease intersection genes.The enrichment analysis revealed that Qingfengteng could potentially impact the body's response to lipopolysaccharide,the apoptosis signaling pathway,and other biological processes.It could also have a therapeutic effect on IMN by regulating apoptosis-related pathways,including MAPK and cAMP.The docking results indicate the core targets,including JUN and CASP3,could spontaneously bind with the main active components of Qingfengteng,such as β-sitosterol and razmanine.Conclusions Qingfengteng has the potential to regulate multiple signaling pathways including MAPK by targeting crucial elements such as CASP3 and JUN.This regulation leads to controlled apoptosis of inflammatory cells,inhibition of inflammatory factors,and may play a vital role in IMN treatment.
作者
江宜宏
郑佳新
JIANG Yihong;ZHENG Jiaxin(Heilongjiang Academy of Traditional Chinese Medicine,Harbin 150036,China)
出处
《健康研究》
CAS
2023年第6期672-677,共6页
Health Research
基金
黑龙江省中医药科研项目(ZHY2020-049)。
关键词
青风藤
网络药理学
GEO芯片
特发性膜性肾病
细胞凋亡
Qingfengteng(Sinomenii Caulis)
network pharmacology
GEO chip
idiopathic membranous nephropathy
apoptosis
