摘要
目的:研究并建立紫外分光光度法(UV)和高效液相色谱法(HPLC)分别测定温胆汤物质基准中总黄酮、柚皮苷和新橙皮苷含量。方法:以柚皮苷为对照品,采用紫外-可见分光光度法测定总黄酮的含量并采用HPLC法测定柚皮苷和新橙皮苷的含量。结果:采用直接测定法测定总黄酮类成分在284 nm处有较强吸收,柚皮苷在20.22~80.88μg/mL范围内与吸光度呈良好的线性关系,平均加样回收率为98.33%。柚皮苷和新橙皮苷分别在0.01~0.51 mg/mL和0.01~0.50 mg/mL范围内有良好的线性关系,精密度、稳定性、重复性的RSD均小于5.0%,加样回收率分别为97.11%和102.72%;温胆汤物质基准中总黄酮的平均含量为15.03%,柚皮苷、新橙皮苷的平均含量分别为22.78 mg/g和9.75 mg/g。结论:建立的温胆汤物质基准总黄酮、柚皮苷和新橙皮苷含量测定方法简单、稳定、重复性好,为温胆汤及其相关制剂进一步研究和开发提供实验基础。
Objective:To establish ultraviolet and visible spectrophotometry(UV)and high performance liquid chromatography(HPLC)methods for the determination of total flavonoids,naringin and neohesperidin substance benchmarks of classical famous prescription Wendan Decoction.Methods:With naringin as the reference substance,the contents of total flavonoids were determined by UV.The contents of naringin and neohesperidin were determined by HPLC analysis.Results:Strong absorption was observed at 284 nm by direct determination method.A good linear relationship was obtained for naringin in the concentration range of 20.22~80.88μg/mL,and the average recovery rate was 98.33%.A good linear relationship was observed in the concentration range of 0.01~0.51 mg/mL(r=0.9998)and 0.01~0.50 mg/mL(r=0.9998)for naringin and neohesperidin,respectively.RSDs of precision,stability and reproducibility tests were lower than 5.0%. The average recovery rates were 97.11% and 102.72%. Conclusion:The methodsfor content determination of total flavonoids, naringin and neohesperidin are simple, stable and repeatable,which provided experimental basis for the further research and development of Wendan Decoctionand its related formulation.
作者
杨思雨
邹妍
李寅庆
张红梅
Yang Siyu;Zou Yan;Li Yinqing;Zhang Hongmei(School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery,Shanghai 201203,China;Shineway Pharmaceutical Group Ltd.,Shijiazhuang 051430,China;Hebei Key Lab.of Famous Classical Formulas and TCM Quality Process Control,Shijiazhuang 051430,China)
出处
《中国野生植物资源》
CSCD
2023年第10期30-36,共7页
Chinese Wild Plant Resources
基金
广东省重点领域研发计划资助(2020B1111110003)
2020教育部新农科研究与改革实践项目
2020上海市高校本科重点教改项目。
关键词
温胆汤
总黄酮
柚皮苷
新橙皮苷
Wendan Decoction
Total flavonoids
Naringin
Neohesperidin
