摘要
目的通过生物信息学分析筛选出可以早期诊断及判断胃癌预后的生物标志物。方法从GEO数据库获得GSE数据集,使用GEO2R在线工具筛选出差异表达基因(DEGs),应用基因本体论(GO)及京都基因和基因组数据库(KEGG)进行功能及通路富集分析,利用STRING、Cytoscape和cBioPortal分析出蛋白相互作用(PPI)网络、共表达基因及枢纽(Hub)基因。基于Kaplan-Meier分析预后生存曲线,使用HPA、Onco mine、UALCAN分析Hub基因在胃癌组织中的表达情况。结果本研究共得到26个Hub基因,它们主要富集在O-聚糖(O-glycan)生物过程。Hub基因中生存分析显示B3GNT6和GATA6的GC患者总生存期较差。在TCGA中,B3GNT6在胃癌组织中表达明显下调,而GATA6表达无显著性差异。结论B3GNT6可能作为潜在的胃癌早期诊断及判断预后的生物标志物。
Objective To screen biomarkers for early diagnosis and prognosis of gastric cancer by bioinformatics analysis.Methods GSE data sets were obtained from GEO database.Differentially expressed genes(DEGs)were screened using the GEO2R online tool.Gene Ontology(GO)and Kyoto Gene and Genome Database(KEGG)were used for functional and pathway enrichment analysis.Protein-protein interaction networks(PPI),co-expressed genes and Hub genes were analyzed using STRING,Cytoscape and cBioPortal.The prognostic survival curve was analyze based on Kaplan-Meier,and the expression of Hub gene in gastric cancer tissues was analyzed using HPA,Onco mine and UALCAN.Results A total of 26 Hub genes were obtained,which were mainly enriched in the O-glycan biological process.Survival analysis in Hub gene showed poor overall survival in GC patients with B3GNT6 and GATA6.In TCGA,the expression of B3GNT6 was significantly down-regulated in gastric cancer tissues,while the expression of GATA6 had no significant difference.Conclusion B3GNT6 may be a potential biomarker for early diagnosis and prognosis of gastric cancer.
作者
史秋婷
孙铭泽
SHI Qiu-ting;SUN Ming-ze(Department of Oncology,The First Hospital of China Medical University,Shenyang 110001,China)
出处
《解剖科学进展》
CAS
2023年第4期378-382,共5页
Progress of Anatomical Sciences
基金
国家自然科学基金(81901846)。
