摘要
为了评定液相色谱质谱法(LC-MS/MS)测定鸡肉中尼卡巴嗪残留标志物残留量的不确定度,试验依据JJF 1059.1-2012《测量不确定度评定与表示》,结合数学模型和实验过程,分析测量不确定度的来源,评定各因素不确定度的分量。结果表明:鸡肉中尼卡巴嗪残留标志物残留量测定结果为4.2μg/kg,其扩展不确定度为0.46μg/kg,结果表示为(4.26±0.46)μg/kg,k=2。影响LC-MS/MS测定鸡肉中尼卡巴嗪残留标志物残留量测量不确定度的主要因素为标准溶液配制、回收率和标准曲线拟合。该评定模型适用于此方法,可通过选择合格、有效的标准物质、较少标准溶液配制过程,优化前处理方法,选择适合浓度梯度的标准曲线等措施降低不确定度,保证检测结果的准确、可靠。
The uncertainty factors for measuring nicarbazine residue in chicken meat was evaluated using liquid chromatography mass spectrometry(LC-MS/MS)in combination with mathematical model and experimental process according to JJF 1059.1-2012 Evaluation and Expression of Uncertainty in Measurement.Nicarbazine residue in chicken meat was measured to be 4.26μg/kg and the expanded uncertainty was 0.46μg/kg.This result was expressed as 4.26±0.46μg/kg(k=2).The uncertainties of the measurement were mainly derived from preparation of standard solution,recovery and calibration of standard curve.The LC-MS/MS evaluation method was suitable for the uncertainty analysis of nicarbazine residue in chicken meat.In order to ensure the accuracy and reliability of the result,the uncertainty might be reduced by selecting qualified and effective reference materials,decreasing standard solution preparation process,calibrating suitable standard curve and optimizing pretreatment method.
作者
张丽芳
康俊杰
张建雄
郑百芹
刘洋
ZHANG Lifang;KANG Junjie;ZHANG Jianxiong;ZHENG Baiqin;Liu Yang(Tangshan Food and Drug Comprehensive Testing Center,Tangshan 063000,China;Hebei Agricultural Products quality and safety testing Innovation Center,Tangshan 063000,China;Tangshan Institute of Industrial Technology for Functional Agricultural Products;Agricultural Products Quality and Safety Testing Center of RuMinistry of Agriculture and Rural Affairs(Tangshan)Tangshan 063000,China)
出处
《中国动物传染病学报》
CAS
北大核心
2023年第3期184-191,共8页
Chinese Journal of Animal Infectious Diseases
基金
河北省高端人才资助项目
河北省创新能力提升计划项目(20567673H)。
关键词
液相色谱质谱法
鸡肉
尼卡巴嗪残留标志物
不确定度
Liquid chromatography mass spectrometry
chicken meat
nicarbazine residue
uncertainty
