摘要
采用氨基酸分析仪测定幼刺参配合饲料中赖氨酸的含量,并对测定结果的不确定度进行评定。测定结果的不确定度来源主要包括样品称量、样品处理及稀释、标准工作溶液配制、氨基酸分析仪及样品测量重复性。对各不确定度分量进行分析,计算合成标准不确定度和扩展不确定度。幼刺参配合饲料中赖氨酸的质量分数测定结果为(1.15±0.06)%(k=2,置信概率为95%)。不确定度评定结果表明,氨基酸分析仪引入的不确定度占比最大,其次为测量重复性、标准工作溶液的制备以及样品处理和稀释。相比之下,样品称量引入的不确定度相对较小。在测定过程中应严格控制仪器各项性能参数和操作规范性,确保设备处于最佳运行状态,以提高测量结果的准确性和可靠性。
An amino acid analyzer was used to detect the content of lysine in juvenile sea cucumber(Apostichopus japonicus)compound feed,and the uncertainty of the determination results was evaluated.The sources of uncertainty in the determination results mainly included sample weighing,sample processing and dilution,preparation of standard working solution,amino acid analyzer,and the repeatability of sample measurements.Each of these uncertainty components was evaluated to calculate the combined standard uncertainty and expanded uncertainty.The determined mass fraction of lysine in the tested juvenile sea cucumber compound feed was 1.15%,with an expanded uncertainty of±0.06%at coverage factor of k=2,representing a 95%confidence level.Contribution analysis of the uncertainty indicated that the uncertainty introduced by the amino acid analyzer accounts for the largest proportion,followed in descending order by measurement repeatability,preparation of standard working solutions,and the steps of sample processing and dilution.In contrast,the uncertainty introduced by sample weighing was relatively minor.During the measurement process,it is necessary to strictly control the performance parameters and operational norms of the instrument to ensure that the equipment is in the optimal operating state,in order to improve the accuracy and reliability of the measurement results.
作者
任召珍
曾海英
李海波
丛文虎
耿建
耿芳芳
于玲
于佳弘
REN Zhaozhen;ZENG Haiying;LI Haibo;CONG Wenhu;GENG Jian;GENG Fangfang;YU Ling;YU Jiahong(Weihai Ocean Vocational College,Rongcheng 264300,China;Shandong Shijin Testing Service Co.,Ltd.,Rongcheng 264300,China;Wendeng District Marine Environment Monitoring Station,Wendeng 264400,China)
出处
《化学分析计量》
2026年第1期140-146,共7页
Chemical Analysis And Meterage
基金
山东省高等学校海洋食品药品资源开发新技术研发中心科研专项资金项目
威海市特色果蔬高值加工工程技术研究中心科研专项资金项目(SDSY20240003)。
关键词
配合饲料
幼刺参
氨基酸分析仪
赖氨酸
不确定度
compound feed
juvenile sea cucumbers
amino acid analyzer
lysine
uncertainty
