摘要
以“辽宁1号”“新新2号”“新早丰”“中林3号”“薄丰”“晋龙1号”“孝核1号”“扎343”和“鲁光”9个核桃品种的一年生休眠期枝条为试材,采用人工模拟降温胁迫处理的方法,研究不同低温胁迫(4、-20、-25、-30、-35、-40℃)处理对9种核桃枝条相对电导率(REC)、可溶性蛋白质(SP)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等生理生化指标的影响,并采用隶属函数法对各核桃品种的抗寒性生理指标进行综合评价,以期为筛选适宜青海地区栽培的抗寒性强的核桃品种提供参考依据。结果表明:随着胁迫温度的降低,9个核桃品种的相对电导率呈逐渐上升的变化趋势,可溶性蛋白质、可溶性糖、CAT、SOD、POD的变化趋势存在一定的差异;通过隶属函数法对9个核桃品种进行抗寒性强弱排序,由高到低依次为“辽宁1号”>“中林3号”>“孝核1号”>“新早丰”>“晋龙1号”>“鲁光”>“薄丰”>“扎343”>“新新2号”。
The one-year-old dormant branches of nine walnut varieties(‘Liaoning 1'‘Xinxin 2'‘Xinzaofeng'‘Zhonglin 3'‘Baofeng'Jin Long 1'‘Xiaohe 1’‘Zha 343'and‘Luguang')were used as test materials,the effects of different low temperature stress conditions(4,-20,-25,-30,-35℃and-40℃)on the physiological and biochemical indexes such as relative electrical conductivity(REC),soluble protein(SP),superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)of nine walnut species were investigated by artificially simulated cooling stress treatments,and the physiological indexes of cold resistance of each walnut species were comprehensively evaluated by the affiliation function method,in order to provide reference for the selection of cold-resistant walnut species suitable for cultivation in Qinghai.The results showed that the relative conductivity of the nine walnut varieties gradually increased as the stress temperature decreased,and there were some differences in the trends of soluble protein,soluble sugar,CAT,SOD and POD.The nine walnut varieties were ranked in order of cold resistance by the subordinate function method,from highest to lowest‘Liaoning 1'>‘Zhonglin 3'>‘Xiaohe 1'>‘Xinzaofeng'>‘Jinlong 1'>‘Luguang'>‘Baofeng'>‘Zha 343'>‘Xinxin 2'.
作者
谢婉莹
祁银燕
刘小利
顾文毅
魏海斌
XIE Wanying;QI Yinyan;LIU Xiaoli;GU Wenyi;WEI Haibin(Academy of Agricultural and Forestry Sciences,Qinghai University/Key Laboratory of Genetic Breeding of Highland Forests in Qinghai Province,Xining,Qinghai 810016)
出处
《北方园艺》
CAS
北大核心
2023年第12期29-36,共8页
Northern Horticulture
基金
青海省重点研发与转化计划资助项目(2023-QY-207)
青海省人才托举工程资助项目(2021QHSKXRCTJ02)。
关键词
核桃
抗寒性
隶属函数
综合评价
walnut
cold resistance
subordinate function
comprehensive evaluation
