摘要
目的:探讨骨髓细胞在受致死剂量照射的受体鼠体内的多向分化潜能。方法:将C57-TgN(Mx-cre)雄性小鼠的骨髓细胞经尾静脉植入受致死剂量照射的雌性受体鼠,重建受体鼠骨髓,然后分别用PCR和免疫组化分析检测受体鼠的多个器官组织中sry及Mx-cre基因的分布及表达情况。结果:PCR和免疫组化分析结果均显示,供体细胞的两个标志sry及Mx-cre在受体鼠的肝脏、肾脏、皮肤、脾脏等多个器官中都有表达。结论:骨髓细胞不仅可以重建受致死剂量照射受体鼠的造血系统,而且具有分化为肝脏、肾脏、皮肤、脾脏等组织细胞类型的多向潜能性。
Objective: To observe the multipotent differentiation ability of bone marrow cells in irradiated syngeneic recipient mice. Methods: Marrow cells isolated from male C57-TgN (Mx-Cre) mice were transplanted intravenously into irradiated syngeneic female mice to reconstruct their marrow system. The expressions of 2 markers of donor cells, sry and Mx-cre gene, were detected by PCR and immunohistochemistry in several organs of recipient mice. Results: Both sry and Mx-cre were positive by PCR detection in liver, kidney, skin and spleen of recipient mice. Immunohistochemistry outcomings were coincident with those of PCR. Conclusion: The results above suggest that marrow cells can not only reconstruct the hematopoiesis system of irradiated syngeneic mice, but also show multipotency of differntiating into histocytes of liver, kidney, skin and spleen.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2002年第11期1172-1174,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目(30270668)
国家教育部高等学校骨干教师资助项目.
关键词
骨髓细胞
造血干细胞
细胞分化
marrow cells
hematopoietic stem cells
cell differentiation
