摘要
目的探讨不同严重程度溃疡性结肠炎(UC)患者机体炎症状态和Toll样受体(TLRs)表达与肠道菌群紊乱的关系。方法选取2021年1月至2022年1月我院诊治的102例UC患者作为研究组,以同期50例体格检查健康者作为对照组,对两组受试者的肠道菌群进行检测,同时检测患者TLR2、TLR4、TLR5、TLR9以及C反应蛋白(CRP)、白细胞介素(IL)6表达水平。指标间的关系采用Pearson相关性分析。结果研究组患者肠道双歧杆菌、嗜酸乳杆菌、拟杆菌数量均低于对照组,大肠埃希菌、肠球菌数量均高于对照组,且研究组中活动期患者肠道双歧杆菌、嗜酸乳杆菌、拟杆菌数量低于缓解期患者,而大肠埃希菌、肠球菌数量均高于缓解期患者(均P<0.05)。研究组患者TLR2、TLR4、TLR5、TLR9相对表达量均高于对照组,且研究组中活动期患者TLRs相对表达量均高于缓解期患者(均P<0.05)。研究组患者CRP、IL-6水平均高于对照组,且研究组中活动期患者CRP、IL-6水平均高于缓解期患者(均P<0.05)。Pearson相关性分析结果显示,不同疾病严重程度UC患者机体炎症状态和TLRs表达量均与肠道菌群失调具有显著相关性(均P<0.05)。结论UC患者肠道菌群表现为优势菌群减少,致病菌群增多。肠道菌群失调、TLRs异常表达、炎症状态与UC的发生和疾病严重程度具有相关性。
Objective To investigate the relationship of intestinal flora disturbance with inflammatory state and expression of Toll-like receptors(TLRs)in patients with ulcerative colitis(UC)of different disease severity.Methods A total of 102 UC patients diagnosed and treated in our hospital from January 2021 to January 2022 were selected as the observation group,and 50 healthy individuals were selected as the control group.The intestinal flora of both groups of subjects were detected.The counts of intestinal flora was counted,and the expression levels of TLR2,TLR4,TLR5,TLR9,C-reactive protein(CRP)and interleukin(IL)6 were detected.The relationships were analyzed using Pearson correlation.Results The counts of intestinal Bifidobacteria,Lactobacillus and Bacteroides the study group were lower than those in the control group,while the counts of E.coli and Enterococcus were higher than those in the control group.Additionally,the counts of intestinal Bifidobacteria,Lactobacillus,and Bacteroides in the active phase patients were lower than those in the remission phase patients,while the counts of E.coli and Enterococcus were higher than those in the remission phase patients.There were statistical differences in the counts of intestinal flora between the two groups(all P<0.05).The relative expression levels of TLR2,TLR4,TLR5 and TLR9 in the observation group were higher than those in the control group;that of TLRs in active patients in the observation group was higher than that in remission patients(all P<0.05).Pearson correlation analysis showed that the inflammatory state and TLRs expression in UC patients with different disease severity interacted with intestinal flora disorder(all P<0.05).Conclusion UC patients manifest decreased dominant flora and increased pathogenic flora Intestinal flora disorder,abnormal expression of TLRs,and inflammatory state are related to the occurrence and severity of UC.
作者
薛小春
田尧
俞春梅
郑志存
XUE Xiao-chun;TIAN Yao;YU Chun-mei;ZHENG Zhi-cun(Department of Gastroenterology,Hai'an People's Hospital,Hai'an,Jiangsu 226600,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2023年第3期344-347,352,共5页
Chinese Journal of Microecology
