摘要
目的 探讨别旁茶是否通过调控小窝蛋白-1(caveolin-1,CAV1)的表达影响炎症性肠病(IBD)细胞模型的增殖和凋亡。方法 采用RT-qPCR和蛋白免疫印迹验证CAV-1-siRNA的敲低效率和pcDNA-CAV-1的过表达效率。采用CCK-8测定别旁茶处理对各组HT-29细胞的增殖影响。蛋白免疫印迹检测别旁茶处理对各组凋亡相关蛋白的表达。蛋白免疫印迹检测pcDNA-CAV-1和别旁茶处理对Occludin和Claudin-1的蛋白表达水平的影响。结果 在HT-29细胞中CAV-1-siRNA的敲低效率和pcDNA-CAV-1的过表达效率得到验证。别旁茶可以通过上调CAV-1促进IBD细胞模型中HT-29细胞的增殖水平和抗凋亡蛋白的表达。别旁茶可以通过上调CAV-1来促进Occludin和Claudin-1的蛋白表达水平。结论 别旁茶通过上调CAV-1来促进Occludin和Claudin-1的蛋白表达水平,从而促进HT-29细胞的增殖水平并抑制其凋亡水平,在IBD细胞模型中发挥保护作用。
Objective To explore whether Bie Pang Cha(BPC,Elongate Jasmine twig and leaf) affects the proliferation and apoptosis of inflammatory bowel disease cell model by regulating the expression of Caveolin-1(CAV-1). Methods RT-qPCR and Western Blot were used to verify the knockdown efficiency of CAV-1-siRNA and the overexpression efficiency of pcDNA-CAV-1. CCK-8 was used to determine the effect of Jasmine on the proliferation of HT-29 cells in each group. Western Blot method was used to detect the expression of apoptosis-related proteins in each group treated with Jasmine. The effects of pcDNA-CAV-1 and Jasmine on the protein expression levels of Occludin and Claudin-1 were detected by Western Blot method. Results The knockdown efficiency of CAV-1-siRNA and the overexpression efficiency of pcDNA-CAV-1 were verified in HT-29 cells. Jasmine can promote the proliferation level and the expression level of anti-apoptotic proteins of HT-29 cells in the inflammatory bowel disease cell model by up-regulating CAV-1. Jasmine can promote the protein expression levels of Occludin and Claudin-1 by up-regulating CAV-1. Conclusion Jasmine promotes the protein expression levels of Occludin and Claudin-1 by up-regulating CAV-1, thereby promoting the proliferation level and inhibiting the apoptosis level of HT-29 cells, playing a protective role in the inflammatory bowel disease cell model.
作者
许研
李思荃
叶展晖
龙友红
徐舒
陈科全
周碧瑶
XU Yan;LI Si-quan;YE Zhan-hui;LONG You-hong;XU Shu;CHEN Ke-quan;ZHOU Bi-yao(Department of Gastroenterology,Guangzhou Cadre Health Management Center,Guangzhou,Guangdong,510530;Department of Gastroenterology,Nanfang Hospital of Southern Medical University,Guangzhou,Guangdong,510515;Department of TCM,Shenzhen Hospital,University of Chinese Academy of Sciences,Shenzhen,Guangdong,518106;Department of Oncology,Shenzhen Hospital,University of Chinese Academy of Sciences,Shenzhen,Guangdong,518106;Department of Gastroenterology,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong,510160;Department of Periodontology,Stomatological Hospital,School of Stomatology,Southern Medical University,Guangzhou,Guangdong,510260)
出处
《现代消化及介入诊疗》
2022年第11期1402-1407,共6页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
广州市科技计划项目(202102080642)
深圳市光明区科技创新局中医药基础研究项目(2020R01131)。
关键词
小窝蛋白-1
别旁茶
炎症性肠病
增殖
凋亡
Caveolin-1(CAV-1)
Jasmine
Inflammatory Bowel Disease
Proliferation
Apoptosis
