摘要
目的:建立快速筛查药食同源食品中的57种非法添加药物的方法。方法:采用QuEhERS前处理技术对提取样品进行净化处理,运用ACQUITY UPLC BEH C18色谱柱对57种非法添加药物进行分离,乙腈—水作为流动相进行梯度洗脱,质谱电喷雾和多反应监测模式进行检测分析。结果:建立的方法线性良好,57种非法添加药物的相关系数均大于0.99,检出限为0.03~0.10μg/kg,平均回收率为79.4%~92.6%,相对标准偏差为0.86%~5.61%。结论:该方法稳定、灵敏度高,适用于快速定性和定量筛查药食同源食品中的57种非法添加药物。
Objective:This study aimed to establish a rapid screening method for 57 drugs illegally added to medicinal and edible food.Methods:Extracted samples were purified by QuEhERS pretreatment technology,and 57 kinds of illegal addition of drugs were separated by Waters BEH C18column.Acetonitrile-water was used as a mobile phase for gradient elution.Mass spectrometry electrospray and multiple reaction monitoring(MRM)were used for detection and analysis.Results:The established method had good linearity,and the correlation coefficients(R~2)of 57 kinds of illegal addition of drugs were all greater than 0.99.The limits of detection were 0.03~0.10μg/kg.The average recoveries were 79.4%~92.6%,and the relative standard deviations(RSDs)were 0.86%~5.61%.Conclusion:The method is stable and sensitive and suitable for rapid qualitative and quantitative screening of 57 kinds of illegally added drugs in medicinal and edible food.
作者
綦艳
许庆鹏
李聪
邓幸飞
李锦清
QI Yan;XU Qing-peng;LI Cong;DENG Xing-fei;LI Jin-qing(Guangdong Testing Institute of Product Quality Supervision,Foshan,Guangdong 528300,China)
出处
《食品与机械》
北大核心
2022年第12期37-43,55,共8页
Food and Machinery
基金
广州市科技计划项目(编号:202102080652)。
关键词
超高效液相色谱—串联三重四极杆质谱法
药食同源食品
非法添加
药物
ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry
medicinal and edible food
illegal addition
drugs
