摘要
目的建立一种同时测定蒲公英中绿原酸、菊苣酸、咖啡酸、木犀草素含量的高效液相色谱方法。方法采用InertSustain^(TM) ODS-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%乙酸水溶液,梯度洗脱,流速为1 ml/min,柱温为30℃,检测波长为325 nm。结果绿原酸、菊苣酸、咖啡酸、木犀草素在5~100μg/ml范围内线性关系良好,平均加样回收率在99.83%~102.05%之间;检测限分别为0.11,0.09,0.06,0.07 ng,定量限分别为0.36,0.30,0.19,0.24 ng;精密度、稳定性、重复性、中间精密度试验的RSD均小于5%,且具有良好耐用性。结论本方法简单高效、准确可靠,可用于蒲公英的质量控制。
Objective To establish an HPLC method for the determination of chlorogenic acid,cichoric acid,caffeic acid and luteolin in Taraxaci Herba at the same time.Methods The chromatographic separation was achieved on an InertSustain^(TM)ODS-C18column(250 mm×4.6 mm,5μm),using acetonitrile-0.1%acetic acid solution at the flow rate of 1.0 ml/min as mobile phase for gradient elution.The column temperature was 30℃and the detection wavelength was set at 325 nm.Results Chlorogenic acid,cichoric acid,caffeic acid and luteolin showed good linear relationship at the ranges of 5-100μg/ml,and the average recoveries were 99.83%-102.05%.The detection limits were 0.11 ng,0.09 ng,0.06 ng,0.07 ng,respectively;and the quantitation limits were 0.36 ng,0.30 ng,0.19 ng,0.24 ng,respectively.RSDs of precision test,stability test,repeatability test and intermediate precision test were all lower than 5%,and the durability was good.Conclusion This method is simple and reliable,so it can be used for the quality control of Taraxaci Herba.
作者
卢秋红
孙梦秋
包明
刘嘉璐
冯春来
LU Qiu-hong;SUN Meng-qiu;BAO Ming;LIU Jia-lu;FENG Chun-lai(Zhenjiang First People’s Hospital,Zhenjiang 212000,China;School of Pharmacy,Jiangsu University,Zhenjiang 212013,China)
出处
《食品与药品》
CAS
2023年第1期85-88,共4页
Food and Drug
基金
国家自然科学基金(编号:82074286)
江苏省“六大人才高峰”高层次人才(编号:SWYY-013)
江苏省自然科学基金(编号:BK20191428)
江苏省中医药管理局中医药科技发展专项(编号:2020ZX21)。
