摘要
目的：建立HPLC同时测定蒲地蓝消炎口服液中7个成分（黄芩苷、汉黄芩素、绿原酸、咖啡酸、菊苣酸、紫堇灵和腺苷）的含量。方法：Phenomenex Luna C18色谱柱（4．6mm×250mm，5μm）；流动相甲醇-0．1％三氟乙酸水溶液，梯度洗脱；检测波长280nm；柱温30℃；流速1．0mL·min^-1。结果：7个被测成分在各自测定的线性范围内线性关系良好，r≥0．9996；平均加样回收率为98．73％～102．1％，RSD均小于2．6％。结论：方法稳定可靠、简便易行，可用于蒲地蓝消炎口服液的质量控制。
Objective： To establish an HPLC method for the content determination of baicalin, wogonin, chlorogenie acid, caffeie acid, eichorie acid, corynoline and adenosine in Pudilan Xiaoyan oral liquid. Method： The analysis was performed on a Phenomenex Luna C18 column （4. 6 mm ×250 mm, 5 μm） with a gradient mobile phase of methanol-0. 1% trifluoroacetic acid solution system at flow rate of 1.0 mL · min^-1. The detective wavelength was at 280 nm. The column temperature was 30 ℃. Result： The standard curves of seven studied components show good linearity in their concentration ranges with r ≥ 0. 999 6. The average recovery was 98.73%-102. 1% with RSD less than 2.6%. Conclusion： The method is rapid, simple and accurate, and can be applied for the quality control of Pudilan Xiaoyan oral liquid.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第9期1747-1750,共4页
China Journal of Chinese Materia Medica
关键词
蒲地蓝消炎口服液
黄芩苷
汉黄芩素
绿原酸
咖啡酸
菊苣酸
紫堇灵
腺苷
HPLC
含量测定
Pudilan Xiaoyan oral liquid
baicalin
wogonin
chlorogenic acid
caffeic acid
cichoric acid
corynoline
adenosine
HPLC
content determination
