摘要
目的 分析良附滴丸中挥发性成分组成,并测定其含量。方法 应用气相色谱-三重串联四极杆质谱(GC-MS/MS)法,根据精确相对分子质量、碎片离子推测结构,通过对照品比对确定成分组成,基于SIM扫描模式进行含量测定。结果 共鉴定出76种挥发性成分,占挥发油总量的83.02%,确认了11种成分[桉叶油醇、D-樟脑、芳樟醇、(-)-4-萜品醇、α-松油醇、苯丙醛、桃金娘烯醇、苄基丙酮、石竹素、香附烯酮、α-香附酮],在各自范围内线性关系良好(r>0.990 0),平均加样回收率95.24%~104.36%,RSD 1.50%~2.97%。结论 该方法准确可靠,可用于良附滴丸中挥发性成分的质量评价。
AIM To analyze the composition of volatile constituents in Liangfu Dropping Pills and to determine their contents.METHODS Applying gas chromatography tandem triple quadrupole mass spectrometry(GC-MS/MS) method, the structures were inferred according to accurate relative molecular weights and fragment ions, the component compositions were determined by comparison of reference substances, the content determination was performed based on SIM scanning mode.RESULTS Total 76 volatile constituents were identified, accounting for 83.02% of the total amount of volatile oils, 11 of which were confirmed [eucalyptol, D-camphor, linalool,(-)-4-terpineol, α-terpineol, benzenepropanal,(-)-myrtenol, benzylacetone, caryophyllene oxide, cyperenone, α-cyperone], which showed good linear relationships within their own ranges(r>0.990 0), whose average recoveries were 95.24%-104.36% with the RSDs of 1.50%-2.97%.CONCLUSION This accurate and reliable method can be used for the quality evaluation of volatile constituents in Liangfu Dropping Pills.
作者
廖永娥
潘洁
陆苑
刘春花
孙佳
汪洋
李勇军
LIAO Yong-e;PAN Jie;LU Yuan;LIU Chun-hua;SUN Jia;WANG Yang;LI Yong-jun(Engineering Research Center for Development and Application of Ethnic Medicine and TCM/State Key Laboratory for Functions and Applications of Medicinal Plants Co-founded by Guizhou Province and MOST,Guizhou Medical University,Guiyang 550004,China;College of Pharmacy,Guizhou Medical University,Guiyang 550004,China;Guizhou Provincial Key Laboratory for Pharmaceutics,Guizhou Medical University,Guiyang 550004,China)
出处
《中成药》
CAS
CSCD
北大核心
2023年第2期375-380,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(82060705)
贵州省中医药、民族医药科学技术研究专项课题项目(QZYY-2020-006)
贵阳市科技计划项目(筑科合同药04号)
贵州医科大学学术新苗培养及创新探索专项项目(黔科合平台人才[2018]5779-11)。
关键词
良附滴丸
挥发性成分
组成分析
含量测定
气相色谱-三重串联四极杆质谱(GC-MS/MS)
Liangfu Dropping Pills
volatile constituents
composition analysis
content determination
gas chromatography tandem triple quadrupole mass spectrometry(GC-MS/MS)
