摘要
为鉴定控制谷子抽穗的关键基因,通过甲基磺酸乙酯(ethyl methylsulfonate,EMS)诱变谷子参考基因组测序品种豫谷1号,获得遗传稳定的谷子超早抽穗突变体jt1242-14b.与豫谷1号相比,jt1242-14b抽穗期明显提前,茎秆变细,叶片变窄、变短.遗传分析表明,突变性状受隐性单基因控制.以SSR41(父本)、jt1242-14b(母本)和F2群体进行突变基因定位,结果表明,该基因位于第9号染色体,在分子标记In4746和In9-11之间的4447 kb之内.进一步测序比对分析发现突变位点位于PHYB基因内部,因此,PHYB很可能是该早熟突变体的目标基因.本研究为谷子早抽穗基因克隆及PHYB基因功能研究提供材料基础.
To identify the target genes controlling heading date,an early heading mutant jt1242-14b was identified from the ethyl methyl sulfonate(EMS)induced mutant library of the foxtail millet variety Yugu1.Compared with Yugu1,jt1242-14b showed an early heading date,thinner stem,and narrow and short leaves.Genetic analysis showed that the mutant was controlled by a recessive gene.Genetic mapping of the mutant gene was conducted from segregating population of jt1242-14b/SSR41.The gene was mapped in an interval of 4447 kb between the molecular marker In4746 and In9-11 on chromosome 9.Finally,jt1242-14b was caused by a mutation in PHYB gene revealed by sequencing,so PHYB could be the target gene of the early heading mutant.This study provided a material foundation for cloning and functional research of PHYB in millet early heading genes.
作者
王春芳
祁东梅
史慎奎
张温典
智慧
刁现民
WANG Chunfang;QI Dongmei;SHI Shenkui;ZHANG Wendian;ZHI Hui;DIAO Xianmin(College of Biological and Food Science,Hebei Normal University for Nationalities,Chengde 067000,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2023年第1期87-94,共8页
Journal of Hebei University(Natural Science Edition)
基金
河北省高等学校科学技术研究项目(ZD2022151,QN2022188)
2021年度河北民族师范学院青年基金项目(QN2021002)
2021年度承德市科技局研究项目(202109A196)。
关键词
谷子
抽穗期
遗传分析
基因定位
Setaria italica
heading date
genetic analysis
genetic mapping
