摘要
以红旗16为受体、明恢63为供体,经杂交和回交得到BC4F1。利用643对SSR分子标记对所构建的抽穗期基因早晚池和亲本进行多态性筛选,得到4对多态性标记。将筛选出的杂合单株进行自交,获得BC4F2,并从中筛选出抽穗期分离较明显的群体进行田间表型调查和基因型检测。发现目标基因与标记PSM391连锁,位于第7染色体长臂末端,命名为Hd7m。在标记PSM391与第7染色体末端之间合成10对新SSR标记,其中多态性标记RM22156与目标基因相距4.1 c M。该结果为Hd7m基因的精细定位、基因克隆和分子标记辅助育种奠定了基础。
Using Hongqi 16 as the receptor and Minghui 63 as the donor,a BC4F1 segregation population was developed through cross between the two parents and backcross with Hongqi 16. The early heading gene pool,late heading gene pool,and the parents were analyzed with 643 pairs of SSR markers,and 4 pairs of polymorphic markers were obtained. BC4F2 populations derived from inbreeding of BC4F1 hybrid plants with obvious segregation phenotype were selected for field investigation and genotype detection. The results showed that the target gene,named Hd7 m,was linked to SSR marker PSM391,which located at the end of chromosome 7. Based on the targeted interval,10 pairs of new SSR markers were synthesized. Among which,the polymorphic marker RM22156 was apart from Hd7 m of 4. 1 c M. The results laid foundations for fine mapping,gene cloning and molecular marker assisted breeding of Hd7 m.
出处
《山东农业科学》
2015年第1期10-13,共4页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31171529)
山东省农业生物资源创新利用课题
山东省农业科学院科技创新重点项目(2014CXZ10-2)
山东省现代农业产业技术体系(水稻)项目资助
