摘要
葡萄糖水平是糖尿病诊断治疗的一项重要评价指标。该文基于激光诱导石墨烯/酶电极构建了一种新型便携式葡萄糖传感器。通过激光诱导石墨烯(LIG)技术制备微型柔性石墨烯三电极系统(LIG电极),并基于1-芘丁酸的疏水端苯环与石墨烯的六元环结构间的π-π叠加效应,对LIG电极表面羧基化,进而共价交联葡萄糖氧化酶(GOx),制得LIG/GOx电极,采用计时电流法,开展葡萄糖传感研究。结果表明,所构建的传感器对葡萄糖的检测范围为5.0×10-5~1.0×10-2mol/L,检出限(S/N=2)为5.0×10-5mol/L。方法表现出较高的选择性、稳定性和良好的重复性,可用于血清、尿样和汗液中葡萄糖的检测。LIG在葡萄糖传感微型电极材料的应用,有利于实现葡萄糖的非临床检测,有望用于便携式传感器的构建。
Glucose level is an important evaluation index for the diagnosis and treatment of diabetes.In this paper,a novel portable glucose sensor was constructed based on laser-induced graphene/enzyme electrodes.Micro-flexible graphene three-electrode system(LIG electrode) was prepared by laser-induced graphene(LIG) technique.The effect ofπ-πsuperposition occurred between the hydrophobic terminal benzene ring of 1-pyrene butyric acid and the six-membered ring structure of graphene introduced the carboxyl onto the LIG electrode,then the glucose oxidase(GOx) was covalently cross-linked to the surface of LIG for preparation of LIG/GOx electrodes.Glucose sensing was carried out by chronoamperometry.Results showed that the constructed sensor could be used to detect glucose in the range of 5.0×10-5-1.0×10-2mol/L with a limit of detectton(S/N=2) of 5.0×10-5mol/L.The method showed high selectivity,stability and good reusability,and could be used to detect glucose in serum,urine and sweat samples.LIG is a micro-electrode material for glucose sensing with good development prospects,which is conducive to the realization of non-clinical glucose detection and is expected to be used in the construction of portable sensors.
作者
陈芳如
张兆康
何书涵
余羲溪
李艳霞
CHEN Fang-ru;ZHANG Zhao-kang;HE Shu-han;YU Xi-xi;LI Yan-xia(College of Materials and Chemical Engineering,Minjiang University,Fuzhou 350108,China;College of Chemical Engineering,Fuzhou University,Fuzhou 350108,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2023年第2期150-157,共8页
Journal of Instrumental Analysis
基金
福建省自然科学基金项目(2020J01835)
国家级大学生创新创业训练计划项目(20221039016)
闽江学院校长基金(103952022144)。
