摘要
Optical microscopy allows us to observe the biological structures and processes within living cells.However,the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light di®raction.Structured illumination microscopy(SIM),a type of new emerging super-resolution microscopy,doubles the spatial resolution by illuminating the specimen with a patterned light,and the sample and light source requirements of SIM are not as strict as the other super-resolution microscopy.In addition,SIM is easier to combine with the other imaging techniques to improve their imaging resolution,leading to the developments of diverse types of SIM.SIM has great potential to meet the various requirements of living cells imaging.Here,we review the recent developments of SIM and its combination with other imaging techniques.
基金
This study was partly supported by the National Key Basic Research Program of China (973 Program)under Grant No.2015CB352006
the National Natural Science Foundation of China under Grants Nos.61335011 and 61405035
Program for Changjiang Scholars and Innovative Research Team in University under Grant No.IRT 15R10.
