摘要
目的:观察电针心包经穴对大脑中动脉闭塞(MCAO)大鼠神经功能缺损、缺血脑组织D-丝氨酸(D-Ser)含量、NMDAR表达的影响。方法:将36只SD大鼠随机分为正常组、假手术组、模型组、电针心包经组、模型+D-Ser组、电针心包经+D-Ser组。采用Zea Longa法制备MCAO模型,模型+D-Ser组、电针心包经+D-Ser组在插入线栓成功后30 min予外源性D-Ser试剂(160 mg/kg i.p.);电针心包经组、电针心包经+D-Ser组于次日行电针治疗30 min;其余组仅捆绑固定30 min。在大鼠造模成功后6 h和第2天干预后分别进行2次行为学评定,随后处死取材。应用液相色谱串联质谱法测缺血脑组织D-Ser含量、Western Blot测NR1、NR2A、NR2B的蛋白表达。结果:处理前与正常组、假手术组比,造模后各组的行为学评分均升高(P<0.05);与模型组相比,模型+D-Ser组和电针心包经+D-Ser组的行为学评分均升高(P<0.05)。处理后与模型组相比,电针心包经组差异无统计学意义(P>0.05),模型+D-Ser组和电针心包经+D-Ser组的行为学评分仍升高(P<0.05);与模型+D-Ser组相比,电针心包经+D-Ser组行为学评分无统计学意义(P>0.05)。组内处理前后比较,除正常组和假手术组外,各组行为学评分均略升高,差异无统计学意义(P>0.05)。与模型组相比,模型+D-Ser组的脑组织D-Ser含量及NR1、NR2A、NR2B的蛋白表达显著增加(P<0.05);电针心包经组的NR1、NR2A、NR2B的蛋白表达下降(P<0.05);与模型+D-Ser组相比,电针心包经+D-Ser组的D-Ser含量及NR1、NR2A、NR2B的蛋白表达均下降(P<0.05)。结论:电针心包经穴可降低急性期MCAO大鼠脑组织中D-Ser含量,调控NR1、NR2A、NR2B蛋白表达下降,抑制NMDA受体活性,发挥神经保护作用。
objective:To observe the effect of electroacupuncture at pericardium meridian on neurological deficit,D-serine(D-Ser)content and NMDAR expression in ischemic brain tissue of rats with middle cerebral artery occlusion(MCAO).Methods:A total of 36 SD rats were randomly divided into normal group,sham operation group,model group,EA pericardium meridian group,model+D-Ser group,and EA pericardium meridian+D-Ser group.The MCAO model was prepared by Zea Longa method.The model+D-Ser group and the EA pericardium meridian+D-Ser group were given exogenous D-Ser reagent(160 mg/kg i.p.)30 minutes after the success of the insertion of the suture.EA pericardium meridian group and EA pericardium meridian+D-Ser group were treated with electroacupuncture for 30 min on the next day.The remaining groups were only bound for 30 min.Behavioral evaluation was performed at 6 h after successful modeling and 2 d after intervention,and then the rats were sacrificed.The content of D-Ser in ischemic brain tissue was measured by liquid chromatography tandem mass spectrometry,and the protein expression of NR1,NR2A and NR2B was detected by Western Blot.Results:Before treatment,compared with the normal group and the sham operation group,the behavioral scores of each group increased after modeling(P<0.05);Compared with the model group,the behavioral scores of the model+D-Ser group and the EA pericardium meridian+D-Ser group were increased(P<0.05).After treatment,compared with the model group,there was no significant difference in the EA pericardium meridian group(P>0.05),The behavioral scores of the model+D-Ser group and the EA pericardium meridian+D-Ser group were still increased(P<0.05);Compared with the model+D-Ser group,the behavioral score of the EA pericardium meridian+D-Ser group was not statistically significant(P>0.05).Comparison before and after treatment in the group,except for the normal group and the sham operation group,the behavioral scores of each group were slightly increased,and the difference was not statistically significant(P>0.05).Compared with the model group,the content of D-Ser and the protein expression of NR1,NR2A and NR2B in the brain tissue of the model+D-Ser group were significantly increased(P<0.05);The protein expression of NR1,NR2A and NR2B decreased in EA pericardium meridian group(P<0.05);Compared with the model+D-Ser group,the D-Ser content and the protein expression of NR1,NR2A and NR2B in the EA pericardium meridian+D-Ser group were decreased(P<0.05).Conclusion:EA at pericardium meridian points can reduce the D-Ser content in brain tissue of rats with acute MCAO,regulate the decrease of NR1,NR2A and NR2B protein expression,inhibit the NMDA receptor activity,and play a neuroprotective role.
作者
袁柳媚
夏云
卢小叶
刘蕾
周颖
娄必丹
YUAN Liu-mei;XIA Yun;LU Xiao-ye;LIU Lei;ZHOU Ying;LOU Bi-dan(Hunan University of Traditional Chinese Medicine,Changsha 410007,China;The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China;Guangdong Nanhua Vocational College of Industry and Commerce,Guangzhou 510507,China;Changsha Health Vocational College,Changsha 410100,China)
出处
《海南医学院学报》
CAS
2023年第2期96-101,共6页
Journal of Hainan Medical University
基金
国家自然科学基金(81774421)
湖南省自然科学基金项目(2018JJ2306)
湖南省研究生科研创新课题省级一般项目(CX20200809)。
