摘要
目的研究大鼠肝缺血/再灌注损伤(HI/RI)过程中骨桥蛋白(OPN)的作用。方法将大鼠分为假手术组(sham)和缺血/再灌注(I/R)6、12和24 h组,每组6只。苏木精-伊红染色(HE染色)观察肝组织的形态学变化;比色法检测大鼠血浆中天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)水平及肝组织中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量;RT-qPCR测定肝组织OPN mRNA表达;ELISA测定肝组织OPN含量。结果与sham组相比,HE染色提示I/R后肝细胞有明显的变性和坏死,以I/R 24 h组最为明显;血浆中AST和ALT水平增高,I/R 12 h时最显著(P<0.01);肝组织中MDA含量升高、SOD活力降低,I/R 24 h最显著(P<0.01);肝组织OPN mRNA表达量在I/R各组均有显著升高(P<0.01),OPN含量在I/R 24 h组显著增加(P<0.05)。结论OPN参与了大鼠HI/RI的发生,它可能是评价肝脏损伤的重要指标。
Objective To study the role of osteopontin(OPN)during hepatic ischemia-reperfusion injury(HI/RI)in rats.Methods The rats were divided into sham operation group(sham)and ischemia-reperfusion(I/R)6,12 and 24 h groups,with 6 rats in each group.HE staining was used to observe the morphological changes of liver tissue.The levels of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in plasma,the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in liver tissue were measured by colorimetric method.The expression of OPN mRNA in liver tissue was determined by RT-qPCR.The content of OPN in liver tissue was determined by ELISA.Results Compared with the sham group,HE staining showed obvious degeneration and necrosis of hepatocytes after I/R,which was most obvious in the I/R 24 h group.The plasma levels of AST and ALT increased,most significantly at I/R 12h(P<0.01).The content of MDA in liver tissue increased,and the activity of SOD decreased,which was most significant at I/R 24 h(P<0.01).The expression of OPN mRNA in liver tissue was significantly increased in I/R groups(P<0.01).OPN content was also significantly increased in I/R 24 h group(P<0.05).Conclusions OPN is involved in the pathogenesis of HI/RI in rats and is a potential indicator applied in the evaluation of liver injury.
作者
郭丽苹
苏娟
田新雁
陆丽
胡亚荣
秦燕
GUO Liping;SU Juan;TIAN Xinyan;LU Li;HU Yarong;QIN Yan(School of Basic Medicine,Dali University,Dali 671000,China)
出处
《基础医学与临床》
2023年第1期83-86,共4页
Basic and Clinical Medicine
基金
云南省科技厅地方本科高校(部分)基础研究项目(2017FH00075)
云南省教育厅科学研究基金教师类项目(2018JS410)。
关键词
骨桥蛋白
肝缺血/再灌注损伤
氧化应激
osteopontin
hepatic ischemia-reperfusion injury
oxidative stress
