摘要
目的研究四川马先蒿Pedicularis szetschuanica Maxim.的化学成分及其体外抗乳腺癌活性。方法80%乙醇提取物采用多种色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构;采用MTT法测试各化合物对人乳腺癌MCF-7、MDA-MB-231细胞的增殖抑制作用。结果从中分离得到12个化合物,分别鉴定为argyol(1)、artselaenins A(2)、campsinol(3)、4-hydroxymethylboschnialactone(4)、8-epideoxy loganic acid(5)、山柰酚(6)、8-对羟苄基山柰酚(7)、槲皮素(8)、松脂素(9)、3-甲氧基-4-羟基苯乙醇(10)、腺嘌呤核苷(11)、2-羟甲基-5-羟基吡啶(12)。通过体外乳腺癌细胞增殖抑制活性分析,化合物3对MCF-7细胞的抑制作用较好,IC 50等于(46.5±2.7)μmol/L,化合物8对MDA-MB-231细胞的IC 50值为(14.8±1.1)μmol/L。结论化合物1~6、8、11为首次从四川马先蒿中分离得到。其中化合物7、9、10、12为首次从马先蒿属植物中分离得到。化合物3对MCF-7抑制活性较弱,化合物8对MDA-MB-231抑制活性中等。
AIM To study the chemical constituents from Pedicularis szetschuanica Maxim.,and evaluate the effects on the human breast cancer cell lines MCF-7 and MDA-MB-231.METHODS The 80%ethanol extract from P.szetschuanica was isolated and purified by various chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The cytotoxicities assay was carried out by MTT method.RESULTS Twelve compounds were isolated and identified as argyol(1),artselaenins A(2),campsinol(3),4-hydroxymethylboschnialactone(4),8-epideoxyloganic acid(5),kaempferol(6),8-C-p-hydroxybenzylkaempferol(7),quercetin(8),pinoresinol(9),3-methoxy-4-hydroxy-phenylethanol(10),adenosine(11),2-hydroxymethyl-5-pyridinol(12).Inhibitory activities assay showed that the IC 50 value of compound 3 for the MCF-7 was(46.5±2.7)μmol/L,and compound 8 for the MDA-MB-231 was(14.8±1.1)μmol/L.CONCLUSION Compounds 7、9、10、12 were obtained from genus Pedicularis for the first time,and Compounds 1-6、8、11 were isolated from this plant.Compound 3 exhibited weak inhibition on MCF-7,and Compound 8 exhibited moderate inhibition on MDA-MB-231.
作者
任华忠
胡冬燕
韩广田
李喜安
南泽东
REN Hua-zhong;HU Dong-yan;HAN Guang-tian;LI Xi-an;NAN Ze-dong(Leshan Vocational&Technical college,Leshan 614000,China;Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission,School of Chemistry and Chemical Engineering,North Minzu University,Yinchuan 750021,China)
出处
《中成药》
CAS
CSCD
北大核心
2022年第12期3862-3866,共5页
Chinese Traditional Patent Medicine
基金
四川省中医药管理局科学技术研究专项(2020JC0069)
北方民族大学科研启动项目(2021KYQD35)。
关键词
四川马先蒿
化学成分
分离鉴定
抗乳腺癌活性
Pedicularis szetschuanica Maxim.
chemical constituents
isolation and identification
breast cancer cells
