摘要
目的探究人参养荣汤对阿尔兹海默症小鼠学习记忆损伤的改善作用及其机制。方法72只小鼠随机分为空白组,模型组,多奈哌齐组(0.1 mg/kg),人参养荣汤低、中、高剂量组(1.56、3.12、6.24 g/kg),每组12只。除空白组外,其余各组小鼠每天腹腔注射100 mg/kg D-半乳糖,灌胃10 mg/kg AlCl_(3),2 h后灌胃给予治疗药物。造模后90 d,行为学实验检测小鼠学习记忆能力,采用试剂盒检测小鼠血清、脑组织生化指标,HE染色评估海马组织损伤情况,蛋白免疫印迹法检测海马组织PSD95/NR2B通路蛋白的表达。结果人参养荣汤及多奈哌齐均对小鼠空间学习记忆有明显改善作用,可以缓解小鼠体内氧化应激,升高脑组织乙酰胆碱(Ach)水平,降低乙酰胆碱酯酶(AchE)活性,减轻海马组织损伤,同时上调PSD95、NR2B的蛋白表达。结论人参养荣汤可明显改善阿尔兹海默症小鼠的学习记忆损伤,缓解氧化应激,恢复小鼠脑组织乙酰胆碱系统,其机制可能与调节PSD95/NR2B信号通路有关。
AIM To investigate the effects and mechanisms of Renshen Yangrong Decoction on learning and memory impairment in mice modeling Alzheimer’s disease(AD).METHODS Seventy-two mice were randomly divided into blank group,model group,donepezil group(0.1 mg/kg),and Renshen Yangrong Decoction groups for low-dose,medium-dose and high-dose interventions(1.56,3.12,6.24 g/kg),respectively,with 12 mice in each group.All of the groups,except the blank group,were intragastrically given once a day the therapeutic drugs 2 hours after intraperitoneal injection of 100 mg/kg D-galactose and intragastric administration of 10 mg/kg AlCl 3.90 days after modeling,the mice had their learning and memory ability assessed by behavior test;their biochemical indices levels of serum and brain tissue detected by kit;their hippocampus damage evaluated by HE staining;and their hippocampus protein expressions of PSD95/NR2B pathway detected by Western blot.RESULTS To the mouse models,both Renshen Yangrong Decoction and donepezil significantly improved their spatial learning and memory,alleviated their oxidative stress,increased Ach level in brain tissue,decreased the activity of acetylcholinesterase(AchE),alleviated the hippocampus damage,and simultaneously up-regulated the protein expressions of PSD95/NR2B.CONCLUSION Renshen Yangrong Decoction can significantly improve the learning and memory impairment of mice modeling AD,relieve their oxidative stress,and restore the cerebral acetylcholine system.And the mechanism may be related to the regulation of PSD95/NR2B signaling pathway.
作者
吕馨
蒋蒙蒙
朱梦姚
袁海阳
郭自贺
王祎
贡岳松
Lü Xin;JIANG Meng-meng;ZHU Meng-yao;YUAN Hai-yang;GUO Zi-he;WANG Yi;GONG Yue-song(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《中成药》
CAS
CSCD
北大核心
2022年第12期3823-3829,共7页
Chinese Traditional Patent Medicine
基金
2016年江苏省双创计划项目(013033004004A)
南京中医药大学高级人才科研启动(013074004016)。
