摘要
目的:研究白藜芦醇对脊髓损伤(Spinal cord injury,SCI)小鼠脊髓组织神经元凋亡和凋亡相关蛋白表达的影响。方法:21只雌性C57BL/6小鼠,6-8周龄,随机分为三组:Sham组(假手术对照组),SCI组(脊髓损伤模型)和Resveratrol组(白藜芦醇治疗的脊髓损伤模型),每组7只。通过Basso小鼠量表(BMS)评估小鼠后肢运动功能、HE染色评估小鼠脊髓病变面积、尼氏染色检测脊髓组织神经元数目、TUNEL染色检测凋亡细胞数目。通过酶联免疫试剂盒检测脊髓组织髓过氧化物酶(myeloperoxidase,MPO)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-6和IL-8蛋白表达水平。通过免疫印迹法检测脊髓组织凋亡相关蛋白Fas、FasL、caspase 3和caspase 8表达水平。结果:与Sham组小鼠相比,SCI模型小鼠脊髓组织病变面积和MPO活性均显著增加(P<0.05),但经白芦藜醇治疗后的Resveratrol组SCI小鼠脊髓组织病变面积和MPO活性较SCI组小鼠显著降低(P<0.05)。与Sham组小鼠相比,SCI模型小鼠BMS评分显著降低(P<0.05),但经白芦藜醇治疗后的Resveratrol组SCI小鼠BMS评分较SCI组小鼠显著升高(P<0.05)。与Sham组小鼠相比,SCI模型小鼠脊髓组织神经元丢失和凋亡均显著增加(P<0.05),但经白芦藜醇治疗后的Resveratrol组SCI小鼠脊髓组织病神经元丢失和凋亡较SCI组小鼠显著降低(P<0.05)。与Sham组小鼠相比,SCI模型小鼠脊髓组织Fas、FasL、caspase 3、caspase 8、TNF-α、IL-6和IL-8蛋白表达水平均显著升高(P<0.05),但经白芦藜醇治疗后均显著降低(P<0.05)。结论:白芦藜醇可显著降低脊髓损伤小鼠脊髓组织神经元凋亡,其机制可能与抑制脊髓损伤小鼠脊髓组织炎症和炎症引起的凋亡蛋白表达有关。
Objective:To study the effect of resveratrol on neuronal apoptosis and apoptosis-related protein expression in spinal cord tissue of mice with spinal cord injury.Methods:Twenty-one female C57BL/6 mice,6-8 weeks old,were randomly divided into three groups:Sham group(sham-operated control group),SCI group(spinal cord injury model)and Resveratrol group(resveratrol-treated spinal cord injury model),7 per group.The motor function of hindlimbs of mice was assessed by Basso mouse scale(BMS),the lesion area of mouse spinal cord was assessed by HE staining,the number of neurons in spinal cord tissue was detected by Nissl staining,and the number of apoptotic cells was detected by TUNEL staining.The protein expression levels of MPO,TNF-α,IL-6 and IL-8 in spinal cord tissue were detected by enzyme-linked immunosorbent assay kit.The expression levels of apoptosis-related proteins Fas,FasL,caspase 3 and caspase 8 in spinal cord tissue were detected by western blotting.Results:Compared with the mice in the Sham group,the lesion area and MPO activity of the spinal cord tissue in the SCI model mice were significantly increased(P<0.05).The mice in the SCI group decreased significantly(P<0.05).Compared with the mice in the Sham group,the BMS scores of the SCI model mice were significantly lower(P<0.05),but the BMS scores of the SCI mice in the Resveratrol group after treatment with resveratrol were significantly higher than those in the SCI group(P<0.05).Compared with the mice in the Sham group,the neuron loss and apoptosis in the spinal cord tissue of the SCI model mice were significantly increased(P<0.05),but the neuron loss and apoptosis of the SCI mice in the Resveratrol group treated with resveratrol were significantly increased.Apoptosis was significantly lower than that in the SCI group(P<0.05).Compared with the mice in the Sham group,the protein expressions of Fas,FasL,caspase 3,caspase 8,TNF-α,IL-6 and IL-8in the spinal cord tissue of the SCI model mice were significantly increased(P<0.05),but after After resveratrol treatment,they were significantly decreased(P<0.05).Conclusion:Resveratrol can significantly reduce neuronal apoptosis in spinal cord tissue of mice with spinal cord injury,and its mechanism may be related to the inhibition of spinal cord tissue inflammation and inflammation-induced apoptosis protein expression in spinal cord injury mice.
作者
常乐
段伟
宋瑶
丁子毅
孙振
CHANG Le;DUAN Wei;SONG Yao;DING Zi-yi;SUN Zhen(Department of Orthopedics,Xijing Hospital of Air Force Military Medical University,Xi'an,Shaanxi,710032,China)
出处
《现代生物医学进展》
CAS
2022年第22期4224-4228,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金青年项目(82002348)。
关键词
白芦藜醇
凋亡
神经元
炎症
小鼠
Resveratrol
Apoptosis
Neurons
Inflammation
Mice
