摘要
目的:优化黄芪甲苷纳米乳处方并考察其皮肤滞留量。方法:绘制伪三元相图,确定各相比例。以载药量、平均粒径及多分散指数为评价指标,采用单纯形网格法优化纳米乳处方。采用Franz扩散池法考察皮肤滞留量。结果:优化的处方为丙二醇单月桂酸甘油酯含量5%,混合乳化剂(聚氧乙烯氢化蓖麻油∶1,2-丙二醇,Km=2∶1)含量40%,水含量55%,制备的纳米乳平均粒径为(20.73±0.25)nm,多分散指数为0.183±0.01,载药量为(1.60±0.05)mg·mL^(-1),响应方程预测的各指标值与实测值偏差均小于5%。与黄芪甲苷混悬液比较,纳米乳显著提高黄芪甲苷的皮肤滞留量(P<0.01)。结论:建立的方法预测性好,可用于黄芪甲苷纳米乳处方优化,纳米乳可显著提高黄芪甲苷的皮肤滞留量。
OBJECTIVE To optimize the formulation of astragalosideⅣnanoemulsion and investigate its amount retained in skin.METHODS Pseudo-tertiary phase diagrams were prepared to obtain the concentration ratio of components of the nanoemulsion.Using the drug loading,average particle size and polydispersity index(PDI)as the evaluation indexes,the formulation of nanoemulsion was optimized by simplex lattice method.The skin retentions were investigated by Franz diffusion pool method.RESULTS The optimized formulation was 5%lauroglycol FCC content,40%mixed emulsifier(polyoxyethylene hydrogenated castor oil:1,2-propylene glycol,Km=2∶1)and 55%water.The average particle size was(20.73±0.25)nm,PDI was 0.183±0.01,the drug loading was(1.60±0.05)mg·mL^(-1).The deviation of each index value predicted by the response equation from the measured value was less than5%.Compared with the astragalosideⅣsuspension,the nanoemulsion formulation significantly increased the skin retention of astragaloside IV(P<0.01).CONCLUSION The established method has good predictability and can be used to optimize the formulation of astragalosideⅣnanoemulsion.The nanoemulsion formulation can significantly increase the skin retention of astragalosideⅣ.
作者
陈筱瑜
冯玉天娇
吴少嫒
黄庆德
CHEN Xiao-yu;FENG Yu-tian-jiao;WU Shao-ai;HUANG Qing-de(School of Pharmacy,Fujian Health College,Fujian Fuzhou 350101,China;School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fujian Fuzhou 350122,China)
出处
《中国医院药学杂志》
CAS
北大核心
2022年第20期2121-2126,共6页
Chinese Journal of Hospital Pharmacy
基金
福建省科技引导性项目(编号:2019Y0050)。
关键词
黄芪甲苷
纳米乳
单纯形网格法
皮肤滞留量
astragalosideⅣ
nanoemulsion
simplex lattice method
skin retention
