摘要
目的观察脓毒症肠道功能障碍小鼠肠组织多肽谱差异表达,探索脓毒症肠道功能障碍机制。方法采用盲肠结扎穿孔法建立脓毒症肠道功能障碍小鼠模型,分假手术组(n=3)和脓毒症组(n=3),取回肠组织,运用液相色谱串联质谱法检测肠组织多肽谱,差异表达内源性多肽进行生物信息学分析,推断其在脓毒症肠功能障碍中的作用。结果共鉴定到458条多肽序列,涉及129个前体蛋白,得出差异上调的多肽有101个,下调的多肽有9个,差异内源性多肽可能通过调控代谢途径、抗原处理和呈递、氧化磷酸化、MAPK信号通路、细胞凋亡等参与脓毒症肠道功能障碍的发生发展,并预测出脓毒症肠道功能障碍相关的15条内源性多肽。结论发现了15条差异表达内源性多肽,可能是参与脓毒症肠道功能障碍的关键生物活性多肽,为脓毒症肠道功能障碍的防治提供了新的方向。
Objective To observe differential peptidomics of intestinal tissues in mice with sepsisinduced intestinal dysfunction,and to investigate the mechanism of sepsis-induced intestinal dysfunction.Methods The mice model of sepsis-induced intestinal dysfunction was established by cecal ligation and perforation and divided into sham operation group(3 mice)and sepsis group(3 mice).The peptidomic profiling of intestinal tissue was detected by liquid chromatography-tandem mass spectrometry,and the differentially expressed endogenous peptides were subjected to bioinformatics analysis to assess their possible roles in sepsis-induced intestinal dysfunction.Results A total of 458 peptides and 129 precursor proteins were identified,and 110 of them were differentially expressed,including 101 upregulated peptides and 9 downregulated peptides.Differential endogenous peptides may be involved in the occurrence and development of sepsis-induced intestinal dysfunction through regulation of metabolic pathways,antigen processing and presentation,oxidative phosphorylation,MAPK signaling pathway,cell apoptosis,etc.And 15 endogenous peptides related to sepsis-induced intestinal dysfunction were predicted.Conclusion We found 15 differentially expressed endogenous peptides,which may be the key bioactive peptides involved in sepsis-induced intestinal dysfunction.This study provides a new direction for the prevention and treatment of intestinal dysfunction in sepsis.
作者
陈玉梅
杨依霖
杜施霖
宋振举
童朝阳
CHEN Yu-mei;YANG Yi-lin;DU Shi-lin;SONG Zhen-ju;TONG Chao-yang(Department of Emergency Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China)
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2022年第6期884-890,共7页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金(81800230)
上海市急危重症临床医学研究中心课题(21MC1930400)。
关键词
内源性多肽
差异表达
脓毒症
肠道功能障碍
endogenous peptides
ifferential expression
sepsis
intestinal dysfunction
