摘要
目的通过对芎冰滴丸薄层鉴定和有效成分的含量测定,明确芎冰滴丸的质量标准。方法采用薄层色谱,对比对照药材溶液、供试品溶液和阴性样品溶液,鉴别芎冰滴丸中的川芎和冰片。设计色谱条件为色谱柱Platisil ODS(5μm,250 mm×4.6 mm),甲醇-0.1%磷酸水溶液(50∶50)为流动相,检测波长323 nm,流速为1.0 ml/min,柱温为35℃,进样量为10μl,进行线性范围、精密度、专属性、重现性和RSD的考察,利用高效液相色谱法(HPLC)识别出制剂中的阿魏酸成分,并明确成分占比。结果在薄层色谱中,通过比较能鉴别出芎冰滴丸中的川芎和冰片。阿魏酸进样量在2.12μg/ml~106.00μg/ml范围内,呈良好的线性关系,回归方程为A=0.8249C+0.5818,r=0.9999,方法的专属性、精密度和重现性良好,RSD为98.72%。结论本研究建立的薄层色谱方法操作简便,专属性强,重现性好,可作为芎冰滴丸的质量控制标准。
Objective The quality standard of Xiongbing Dropping Pills was determined by identifying the thin layer and determining the content of active ingredients.Methods Thin layer chromatography was used to identify Chuanxiong and Borneol in Xiongbing Dropping Pills by comparing medicinal herbs solution,test solution and negative sample solution.The chromatographic conditions were designed as follows,the chromatographic column was Platisil ODS(5μm,250 mm×4.6 mm),the mobile phase was methanol-0.1%phosphoric acid solution(50∶50),the detection wavelength was 323 nm,the flow rate was 1.0 ml/min,the column temperature was 35℃,and the injection volume was 10μl.The linear range,precision,specificity,reproducibility and RSD were investigated.The ferulic acid components in the preparation were identified by high performance liquid chromatography(HPLC),and the proportion of components was determined.Results Chuanxiong and Borneol in Xiongbing Dripping Pills could be identified by comparison in Thin layer chromatography.The linear range of ferulic acid was 2.12-106.00μg/ml.The regression equation was A=0.8249C+0.5818,r=0.9999.The method had good specificity,precision and reproducibility.The RSD was 98.72%.Conclusion The thin layer chromatography method established in this study is simple,specific and reproducible,which can be used as the quality control standard for Xiongbing Dropping Pills.
作者
曾自珍
王利胜
ZENG Zizhen;WANG Lisheng(Department of Medicine,the Sixth Hospital Affiliated to Guangzhou Medical University,Qingyuan People's Hospital,Guangdong Province,Qingyuan 511500,China;College of Chinese Pharmacy,Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou 510006,China)
出处
《中国当代医药》
CAS
2022年第31期171-174,179,共5页
China Modern Medicine
关键词
芎冰滴丸
薄层
高效液相色谱法
质量标准
阿魏酸
Xiongbing Dripping Pills
Thin layer
High performance liquid chromatography
Quality standard
Ferulic acid
