摘要
目的通过网络药理学系统分析川归胶囊治疗白癜风的主要活性成分、潜在作用靶点及作用机制,并以此为基础建立川归胶囊的质量标准。方法通过ETCM数据库检索筛选川归胶囊4味中药(川芎、当归、白鲜皮、补骨脂)的活性成分和各活性成分的靶点信息。分别采用GeneCard数据库和OMIM数据库以“Vitiligo”为关键词检索白癜风疾病的靶点。采用维恩图得到川归胶囊治疗白癜风疾病的交集靶点信息。采用String数据库构建潜在靶点的PPI网络图,导入Cytoscape软件优化,根据拓扑分析数据自由度值筛选川归胶囊治疗白癜风疾病的关键靶点和成分。采用DAVID平台对交集靶点进行GO功能和KEGG通路富集分析;采用TLC法对处方中川芎、当归进行定性鉴别,采用HPLC法对川归胶囊中关键活性成分阿魏酸进行含量测定分析。结果川归胶囊4味中药共收集到77种活性成分和352个潜在靶点,共收集到1192个白癜风疾病靶点,药物与疾病共同靶点50个。通过拓扑分析得到二十四烷酸、阿魏酸、亚油酸、豆甾醇、肉豆蔻酸、补骨脂异黄酮、孕烯醇酮、菜油甾醇、谷甾醇、花翠素是川归胶囊的关键活性成分;ALB、AKT1、TNF、INS、IL-6、ESR1、TP53、IL-1β为川归胶囊治疗白癜风的关键靶点。TLC法鉴别川归胶囊结果显示斑点清晰、分离较好且专属性强。含量测定中阿魏酸在浓度为1.77~70.72μg/ml范围内与峰面积呈良好的线性关系(r=0.9999),精密度、稳定性、重复性试验的RSD均小于3%,平均回收率为102.06%,RSD为1.96%(n=6),其平均含量为0.1027 mg/粒。结论本研究通过网络药理学预测川归胶囊核心成分、作用靶点及通路,探究其通过“多成分-多靶点-多通路”治疗白癜风的作用机制;建立核心成分阿魏酸的定性定量方法,该方法简单准确、稳定可靠,为进一步开发川归胶囊提供理论基础和科学依据。
Objective To systematically analyze the main active components,potential targets and mechanism of Chuangui capsule in the treatment of vitiligo by network pharmacology,and to establish the quality standard of Chuangui capsule on this basis.Methods The active components of four main traditional Chinese medicines(Ligusticum chuanxiong,Angelica sinensis,Cortex Psoraleae and Fructus psoraleae)in Chuangui capsule were screened by ETCM database and the target information of each active component was collected.GeneCard database and OMIM database were used to search for the target of vitiligo disease with“Vitiligo”as the keyword.The target information of Chuangui capsule in the treatment of vitiligo was obtained by deweighting the target of vitiligo and Chuangui capsule respectively and making Venn map.The PPI network diagram of potential targets was constructed by String database and optimized by Cytoscape software.The key targets and ingredients of Chuangui capsule in the treatment of vitiligo were selected according to the degree of freedom of topological analysis data.The DAVID platform was used to analyze the GO function and KEGG pathway enrichment of the intersection target.Radix angelicae sinensis and Rhizoma Chuanxiong in the formula were identified by TLC.The content of ferulic acid in the formula was determined by HPLC.Results A total of 77 active components and 352 potential targets were collected from the four Chinese medicines of Chuangui capsule,1192 vitiligo targets were collected,and 50 common therapeutic targets were obtained at the intersection of drug targets and disease targets.Topological analysis showed that the key active components of Chuangui capsules were 24 alkanoic acid,ferulic acid,linoleic acid,stigmasterol,myristic acid,Corylin,pregnenolone,rapeseed sterol,sitosterol and anthocyanin.ALB,AKT1,TNF,INS,IL-6,ESR1,TP53 and IL-1βwere the key targets of Chuangui capsules in the treatment of vitiligo.The spots in TLC were clear with good separation and specificity.The linearity of the calibration curve was good within the range of 1.77~70.72μg/ml for ferulic acid(r=0.9999).The RSDs of precision,stability and reproducibility tests were all lower than 3%.The average recovery was 102.06%,RSD=1.96%(n=6).Its average content was 0.1027 mg per capsule.Conclusion The core ingredients,action targets and pathways of Chuangui capsules can be predicted by network pharmacology,and the mechanism of action of Chuangui capsules in the treatment of vitiligo by“multi-component-multi-target-multi-pathway”can be explored.The qualitative and quantitative method of ferulic acid is established.The method is simple,accurate,stable and reliable.It provides theoretical basis and scientific basis for the further development of Chuangui capsules.
作者
冯诗涵
崔亚玲
高欢
刘彤
许子华
赵庆春
Feng Shihan;Cui Yaling;Gao Huan;Liu Tong;Xu Zihua;Zhao Qingchun(Shenyang Pharmaceutical University,Shenyang 110016,China;General Hospital of Northern Theater Command,Shenyang 110016,China;Liaoning Institute for Drug Control,Shenyang 110031,China)
出处
《实用药物与临床》
CAS
2023年第6期509-515,共7页
Practical Pharmacy and Clinical Remedies
关键词
川归胶囊
白癜风
网络药理学
机制
质量标准
Chuangui capsule
Vitiligo
Network pharmacology
Mechanism
Quality standard
