摘要
文章采用酶底物法(SimPlate法)、EasyDisc法及平皿计数法对水中菌落总数进行检测。通过检测高浓度质控样品与低浓度指控样品发现,SimPlate法与EasyDisc法检测结果的相对误差更小,更接近于真值,结果更为准确;这两种检测结果的相对标准偏差低于平皿计数法,表明SimPlate法和EasyDisc法连续检测的稳定性优于平皿计数法。通过检测实际样品,并对3种方法检测结果进行统计学分析发现,3种方法在统计学上均具有相关性,无显著差异。采用SimPlate法和EasyDisc法测定水中菌落总数具有操作更简便、人为误差较少、稳定性更好等优势,满足了实验室水中菌落总数的检测要求。
In this paper,enzyme substrate method(SimPlate method),EasyDisc method and plate counting method were used to detect the total number of bacterial colonies in water.Results showed that the relative error of SimPlate method and EasyDisc method was smaller and closer to the true value,and the results were more accurate.The relative standard deviation of the two methods was lower than that of plate counting method,indicating that the stability of SimPlate method and EasyDisc method were better than that of plate counting method.The actual samples were tested and the results of the three methods were statistically analyzed.It was found that the three methods were statistically correlated with each other without significant difference.Using SimPlate method and EasyDisc method to determine the total number of bacteria in water has the advantages of simple and convenient of operation,less human error and better stability,which meets the requirements of the total number of bacteria in laboratory.
作者
曹新垲
张琦
尹宝国
张宝华
张雨晨
CAO Xinkai;ZHANG Qi;YIN Baoguo;ZHANG Baohua;ZHANG Yuchen(Water Quality Monitoring Center,Beijing Waterworks Group Co.,Ltd.,Beijing 100012,China)
出处
《净水技术》
CAS
2022年第11期173-178,共6页
Water Purification Technology
关键词
酶底物法
EasyDisc
法
平皿计数法
菌落总数
准确
简便
enzyme substrates method(SimPlate method)
EasyDisc method
plate counting method
total number of bacterial
colonies accuracy simple and convenient
