摘要
针对微生物快速检测的需求,基于生长时间光谱法设计了大肠菌群快速检测仪器.根据大肠菌群指数生长期与大肠菌群浓度的关系来计算大肠菌群初始浓度;采用分光光度法对大肠菌群的指数生长时间进行检测;通过实验确定了625 nm作为分光光度检测波长;设计了基于双积分球的仪器光路结构,提高了仪器对透过率的测量稳定性,采用样品从实验开始时的初始透过率降低至初始透过率的70%时所需时间作为生长时间,显著降低了检测所需时间,对大肠菌群100 cfu/m L的检测,只需要276 min.建立了大肠菌群的生长时间与大肠菌群初始浓度的数学模型.设计实验评价了本方案平行样标准偏差小于12. 61%;与滤膜法进行比对,相关系数0. 979.
A rapid detection instrument for total coliforms was designed based on the time of growth spectrometer method which calculated the initial concentration of total coliforms according to the relationship between the growth period of total coliforms index and the concentration of total coliforms. The exponential growth time of total coliforms was detected by spectrophotometry. The spectrophotometric wavelength was 625 nm. We designed the optical path structure of the instrument based on the double-integrated sphere to improve the stability. The growth time, which decreased 30% from the beginning of the experiment to the transmittance, was reduced significantly. The detection of the 100 cfu/mL of total coliforms required only 276 minutes. A mathematical model was established to clarify the relationship between the coliforms growth time and the initial concentration of total coliforms. The standard deviation of experiment was less than 12.61%. The correlation coefficient was 0.979 compared with the filter membrane method.
作者
袁琨
沈昊阳
贾东芬
冯达
YUAN Kun;SHEN Haoyang;JIA Dongfen;FENG Da(College of Optical and Electronic Technology,China Jiliang University,Hangzhou 310018,China;Hangzhou CHNSpec Technology Co.Ltd,Hangzhou 310034,China;Beijing Liujiaoti Science and Technology Development Co.Ltd,Beijing 101102,China)
出处
《中国计量大学学报》
2018年第3期345-350,共6页
Journal of China University of Metrology
基金
首都食品质量安全保障项目(No.2016YFF0103302)
关键词
生长时间光谱法
分光光度法
快速检测
微生物分析仪
大肠菌群
time of growth spectrometer method
spectrophotometry
rapid detection
microbiological analyzer
total coliforms
