摘要
为测定再造烟叶浆料中有机酸的浓度,利用过滤、离心、在线固相萃取净化和浓缩样品,通过离子色谱阀切换、二元梯度泵清洗再生净化柱,高压泵分离AS11-HC离子色谱柱中的有机酸,建立了同时测定再造烟叶浆料中17种有机酸的在线净化离子色谱法。结果表明:①17种有机酸在0.5~10.0μg/mL范围内线性关系良好,相关系数均大于0.999,方法检出限为0.002~0.400μg/mL,定量限为0.010~0.900μg/mL。②在2、4、6μg/mL 3个加标水平的平均回收率为90.5%~117.1%,相对标准偏差为0.4%~4.4%。17种有机酸日内、日间测定结果的变异系数分别在0.59%~2.57%、0.72%~4.80%之间。该方法操作简便、可靠、重复性好,可用于再造烟叶浆料中有机酸的测定。
In order to quantify the contents of organic acids in reconstituted tobacco slurry,an online purification ion chromatography method for simultaneously analyzing 17 organic acids was established by adopting filtration,centrifugation,online solid phase extraction purification and concentration of samples.The purification column was regenerated by ion chromatography valve switching and binary gradient pump cleaning,and the organic acids were separated in an AS11-HC ion chromatographic column with a high pressure pump.The results showed that:1)The linearities of 17 organic acids were satisfactory in the range of 0.5-10.0μg/mL with the correlation coefficients all higher than 0.999;the limits of detection were 0.002-0.400μg/mL,and the limits of quantification were 0.010-0.900μg/mL.2)The average recoveries at the spiked levels of 2,4 and 6μg/mL were in the range of 90.5%-117.1%,and the relative standard deviations(RSDs)were 0.4%-4.4%.The coefficients of variation for intraand inter-day determination of the 17 organic acids ranged from 0.59%to 2.57%and from 0.72%to4.80%respectively.This method is simple,reliable and repeatable,and can be used for analyzing the organic acids in reconstituted tobacco slurry.
作者
李华雨
李晓瑜
刘宁
薛建中
许红涛
王茹楠
王毅博
王宁
高凡钦
LI Huayu;LI Xiaoyu;LIU Ning;XUE Jianzhong;XU Hongtao;WANG Runan;WANG Yibo;WANG Ning;GAO Fanqin(Key Laboratory for Standardization of Reconstituted Tobacco in Tobacco Industry,Henan Cigarette Industrial Reconstituted Tobacco Sheet Co.,Ltd.,Xuchang 461000,Henan,China;National Meat Product Quality Supervision and Inspection Center,Luohe Quality Technology Supervision and Test Center,Luohe 462000,Henan,China;Enlightenment Puhua Water Co.,Ltd.,Beijing 100084,China;Beijing Application Center,Thermo Fisher Science(China)Co.,Ltd.,Beijing 100102,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2022年第10期44-52,61,共10页
Tobacco Science & Technology
基金
河南中烟工业有限责任公司科技项目“再造烟叶浆料新鲜度表征方法研究”(A202064)。
关键词
再造烟叶
浆料
在线固相萃取
离子色谱法
有机酸
Reconstituted tobacco
Slurry
Online solid phase extraction
Ion chromatography
Organic acid
