摘要
植物光敏色素作用因子(phytochrome interacting factor,PIF)是广泛分布于植物体内的一种转录因子,在植物的生长发育方面有着重要的作用.基于香蕉基因组数据,对香蕉MaPIF基因家族进行基因组鉴定,采用生物信息学分析方法对其进行命名,分析理化性质、蛋白质结构、基因结构、启动子顺势作用元件以及构建系统进化树;分析PIF家族在不同激素处理下的表达情况.结果显示,香蕉MaPIF家族有7个成员,均含有高度保守的bHLH结构域;编码区长度在1 116-2001bp之间,至少包含5个内含子,且大部分位于细胞外;进化树结果可以发现与拟南芥、水稻以及玉米PIF的亲缘关系较近;顺式作用元件预测结果显示,MaPIF上存在多种与激素和光相关的响应元件.qRT-PCR结果显示,MaPIF3-1、MaPIF4、MaPIF4-1在生长素(IAA)、赤霉素(GA)、生长素抑制剂(NPA)处理中均有显著表达,除此之外,所有成员在脱落酸(ABA)处理下均有明显表达.本研究表明MaPIF在香蕉生长发育中激素调控有重要作用.(图7表3参45)
The phytochrome-interacting factor(PIF) is a transcription factor that is widely distributed in plants and plays an important role in the growth and development of plants. Based on banana genome data, in this paper,we identified the banana PIF gene family(MaPIF), named it by using bioinformatics analysis methods, analyzed its physical and chemical properties, protein structure, gene structure, and promoter homeopathic elements,and constructed a phylogenetic tree to analyze the expression of the MaPIF family under different hormone treatments. The results showed that the banana MaPIF family had seven members, all of which contained the highly conserved bHLH and APB domains;the coding regions were between 1 116 and 2 001 bp in length and contained at least five introns, most of which were located outside the cell. The evolutionary tree results showed a close relationship between PIF of banana and those of rice and corn;the prediction results of cis-acting elements indicated that several response elements were related to hormones and light on MaPIF. The qRT-PCR results showed that MaPIF3-1, MaPIF4, and MaPIF4-1 were significantly expressed following treatment with auxin(IAA), gibberellin(GA), and auxin inhibitor(NPA). In addition, all its members were significantly expressed under ABA treatment. The results of this study indicated that MaPIF plays an important role in hormone regulation during banana growth and development.
作者
吴昊
张城瑜
倪珊珊
罗彬彬
王梦鸽
魏雯敏
陈裕坤
赖钟雄
WU Hao;ZHANG Chengyu;NI Shanshan;LUO Binbin;WANG Mengge;WEI Wenmin;CHEN Yukun;LAI Zhongxiong(Institute of Horticultural Plant Bioengineering,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2022年第4期1057-1065,共9页
Chinese Journal of Applied and Environmental Biology
基金
国家重点研发计划项目(2019YFD1000900)
国家现代农业产业技术体系(香蕉)专项资金项目(GARS-31-15)
福建农林大学科技创新专项基金项目(CXZX2013119,CXZX2017351)资助。
