摘要
香蕉杂交品种‘粉杂1号’是当前商业种植中唯一的抗香蕉枯萎病粉蕉(Musa spp. ABB,Pisang awak)品种,该品种来源于高感病的‘广粉1号’(ABB)偶然得到的种子实生苗,其基因组组成和父本未知。通过流式细胞技术(FCM)、染色体制片、基于ITS的限制性片段长度多态性聚合酶链反应(ITS-PCR-RFLP)及反转录转座子间扩增多态性(copia-IRAP)分子标记技术,并结合形态性状评分对‘粉杂1号’基因组组成进行鉴定。流式细胞分析结果显示‘粉杂1号’为四倍体,染色体制片显示染色体数为2n=4x=44条,ITS-PCR-RFLP分子标记结果显示其同时具有A和B基因组,copia-IRAP结果显示其含两套或者以上B基因组;形态打分为67分,对应基因组组成为ABBB。综合以上结果认为‘粉杂1号’基因组组成为ABBB,推测其父本可能为BB基因组组成的野生蕉。
The banana cultivar ’Fenza 1’ is the only Fenjiao(Musa spp. ABB,Pisang awak)cultivar with resistance to Fusarium wilt in the current commercial banana plantings. The cultivar came from the seeds seedling obtained accidently. Its genomic composition and male parent are unknowen. In this study,Flow cytometry( FCM),chromosome flaking, molecular marker technology ITS-PCR-RFLP and copia-IRAP, cooperate with characters taxonomic scorecard were used to identify the genomic composition of ’Fenza 1’. The ploidy was tetraploid showed by the FCM. The chromosome number was 2n = 4x = 44 showed by the chromosome flaking;its genomic composition include A and B showed by the ITS-PCR-RFLP;two or more than two B genomic groups showed by the copia-IRAP;the score of the characters taxonomic is 67,and the corresponding genomic composition is ABBB. Based on the results above,we prosposed that the genomic composition of ’Fenza1’ is ABBB,and speculated its male parent may be wild banana with the genomic composition BB.
作者
杨兴玉
许林兵
吴元立
邱迪洋
范琳琳
黄秉智
YANG Xingyu;XU Linbing;WU Yuanli;QIU Diyang;FAN Linlin;HUANG Bingzhi(Key Laboratory of South Subtropical Fruit,Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affair,Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第9期1991-1997,共7页
Acta Horticulturae Sinica
基金
广东省重点领域研发计划(2021B0707010004-05)
科技部国家重点研发计划(2017YFD0202105)
科技部、财政部国家科技资源共享服务平台(NHGRC2020-NH17)
广东省农业科学院果树研究所自主培育项目(21112)。
关键词
香蕉
杂交种
基因组
分子标记
性状评分
banana
hybrid
genome
molecular marker
characters taxonomic scorecard
