摘要
香蕉枯萎病是影响世界香蕉产业的毁灭性病害。本研究对香蕉枯萎病抗病品种抗枯5号香蕉、粉杂一号粉蕉和感病品种巴西香蕉和广粉一号粉蕉的幼苗根系接种后的球茎组织的进行了超微结构变化观察,结果表明清水对照中4个品种球茎组织的细胞形态和结构正常且完整,细胞代谢强;枯萎病原菌侵染处理下,病菌从根部受损部位侵入,穿过薄壁组织后进入维管组织,球茎木质部导管中相继出现侵填体及一些灰褐色物质,质壁分离;感病品种巴西香蕉和广粉一号粉蕉的球茎细胞壁破损断裂,细胞器肿胀变形,膜溶解呈不完整状态;抗病品种抗枯5号香蕉和粉杂一号粉蕉受害较轻,细胞器膜受损较轻,内部结构基本无破损,其维管细胞壁加厚,皮层薄壁细胞壁木栓化,尤其在抗枯5号香蕉品种中出现乳突的抗病特征。抗病品种的这些防卫反应阻碍了病原菌的进一步入侵及输导。
Abstract Fusarium wilt is a worldwide disastrous banana disease. In this study, ultrastructure observing was performed to understand the disease-resistant mechanism of cell structure alternation in banana via Foe （Fusarium Oxys_porum f. sp. Cubense） inoculation treatment. Banana cultivars with different disease-resistant levels （resistant cultivars： Kangku 5, Musa AAA, and Fenza 1, Musa ABB, and susceptible cultivars： BAXI Cavendish, Musa AAA, and Guangfen 1, Musa ABB）, were inoculated by dipping roots, and any change in cell structure of pseudostem was observed by using electron microscopy. Results showed that plasmolysis, decomposition and rupture of cell wall and membranes of cytoplasmic organoids were observed in the susceptible cultivars, while the breakage in the resistant cultivars was much slight. With the inoculation of Foe4, tylose and brown-like materials in vessels were observed in both types of cultivars, but thickening cuticles and suberification, and tubercles in cell wall were only appeared in the resistant cultivars. These results indicated that ultrastructure alteration of banana pseudostem might be against infection ofFusarium Oxysporum f. sp. Cubense.
出处
《分子植物育种》
CAS
CSCD
北大核心
2013年第2期193-198,共6页
Molecular Plant Breeding
基金
NCSF-广东联合基金(U1131004)
广东省自然科学基金项目(10451064001006121)
广东省农业科学院院长基金(201013)
公益性行业(农业)科技专项(200903049-10)共同资助
关键词
香蕉
枯萎病
尖孢镰刀菌古巴专化型
超微结构
Banana, Fusarium wilt, Fusarium Oxysporum f. sp. Cubense, Ultrastructure
