摘要
目的观察抑郁症患者的记忆性T细胞亚群变化,并探讨不同亚类记忆性T细胞与抑郁严重程度的关系。方法选取2020年10月至2021年10月首都医科大学附属北京安定医院就诊的66例抑郁症患者作为抑郁症组,同时选取年龄、性别匹配的59例健康人作为对照组。采用汉密尔顿抑郁量表(Hamilton depression rating scale 17,HAMD-17)和9项患者健康问卷(patient health questionnaire,PHQ-9)评估患者的抑郁严重程度。通过流式细胞术检测所有受试者的外周血记忆性T细胞亚群,包括中央记忆性T细胞(central memory T cell,Tcm)和效应记忆性T细胞(effector memory T cell,Tem),比较抑郁症组和对照组之间记忆性T细胞亚群的差异,并分析患者记忆性T细胞亚群的比例与抑郁严重程度的关系。结果抑郁症组T细胞、CD4^(+)T细胞和CD8^(+)T细胞比例与对照组相比,差异均无统计学意义(P>0.05)。记忆性T细胞亚群分析显示,抑郁症组CD4^(+)Tcm和CD8^(+)Tcm比例均显著低于对照组[(19.6±16.0)%比(28.8±18.7)%,13.2(4.8,26.8)%比23.8(9.7,38.8)%],CD4^(+)Tem和CD8^(+)Tem比例均显著高于对照组[37.3(19.3,49.2)%比9.7(2.8,30.9)%,28.6(17.1,41.6)%比12.5(2.2,26.5)%],差异有统计学意义(P<0.05)。对照组女性T细胞亚群比例与男性相比,差异无统计学意义(P>0.05),但抑郁症组女性CD8^(+)T细胞比例显著低于男性[(33.6±10.6)%比(41.4±10.6)%],差异有统计学意义(P=0.006)。记忆性T细胞亚群细胞比例与抑郁严重程度评分均无显著相关性(P>0.05)。结论抑郁症患者外周血中Tcm和Tem比例发生相反的变化,提示不同亚型记忆性T细胞在抑郁症中的作用存在差异。
Objective To observe the memory T cell subsets in patients with major depressive disorder(MDD)and explore the relationship between memory T cell subsets and the severity of depression.Methods Sixty-six patients with MDD admitted to Beijing Anding Hospital,Capital Medical University from October 2020 to October 2021 were enrolled in this study,while fifty-nine age and gender-matched healthy individuals were recruited as the control group.The 17-item Hamilton depression rating scale(HAMD-17)and patient health questionnaire(PHQ-9)was used to evaluate the severity of depression of the patients.Flow cytometry was used to detect the memory T cell subsets of peripheral blood in all subjects,including central memory T cells(Tcm)and effector memory T cells(Tem).The differences of memory T cell subsets between MDD patients and healthy controls were compared,and the relationship between the memory T cell subsets and the severity of depression was analyzed.Results There was no significant difference in the proportion of T cells,CD4^(+)T cells or CD8^(+)T cells between MDD patients and the controls(P>0.05).Memory T cell subgroup analysis showed that the proportions of CD4^(+)Tcm and CD8^(+)Tcm in MDD patients were significantly lower than those in healthy controls[(19.6±16.0)%vs.(28.8±18.7)%,13.2(4.8,26.8)%vs.23.8(9.7,38.8)%].However,the proportions of CD4^(+)Tem and CD8^(+)Tem were significantly higher than those in healthy controls[37.3(19.3,49.2)%vs.9.7(2.8,30.9)%,28.6(17.1,41.6)%vs.12.5(2.2,26.5)%,P<0.05].There was no significant gender difference in the proportion of T cell subsets in the healthy subjects(P>0.05),while the proportion of CD8^(+)T cells in women in the depression group was significantly lower than that in men[(33.6±10.6)%vs.(41.4±10.6)%,P=0.006].No significant correlations were found between the proportion of memory T cell subsets and the severity of depression in MDD patients(P>0.05).Conclusions The proportions of Tcm and Tem cells in peripheral blood of MDD patients exhibit inversely alteration,which suggests that memory T cell subsets may play different roles in depression.
作者
李光
张玲
贺毅
孙作厘
Li Guang;Zhang Ling;He Yi;Sun Zuoli(Department of Gastroenterology,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China)
出处
《北京医学》
CAS
2022年第6期475-479,共5页
Beijing Medical Journal
基金
国家自然科学基金(82171525,31970918)。
