摘要
野生菌栽培具有重要的经济价值。本文报道了分离培养野生侧耳属菌株PO1,并进行生物栽培转化的实验结果。以真菌试剂盒提取的PO1基因组DNA为模板,PCR扩增获得了大小为636 bp的ITS序列(GenBank No.MT261808.1)。运用ClustalX和MEGA软件的NJ法构建了侧耳属代表种的系统发育树,结果显示,PO1与NCBI中其他54株侧耳菌株亲缘关系较为密切,相似性达99.04%以上。明确野生侧耳为糙皮侧耳菌株分类后,总结出一套侧耳室内生物栽培转化技术与管理体系,栽培转化的糙皮侧耳PO2头潮菇和二潮菇产量高、口感好,经济效益佳,转化率高达158%,达到实验室侧耳高产栽培的目的,为大棚栽培管理技术提供了重要参考依据。
The cultivation of witd fungi has important economic value.One wild Pleurotus strain was collected for isola-tion,purification and culture,and wild Pleurotus(PO1)was adapted to indoor bag material growth for biological cultivation transformation experiments.The genomic DNA of PO1 was extracted with a fungus kit,and 636 bp ITS sequence was ob-tained by PCR amplification(GenBank accession number MT261808.1).Using the biological analysis software ClustalX and the NJ method in MEGA to construct a phylogenetic tree of the representative species,the results show that PO1 is most close-ly related to other 54 Pleurotus strains,with a similarity of more than 99.04%.After the wild P.ostreatus is classified as P.os-treatus,a set of indoor biological cultivation and transformation technology and management system of P.ostreatus was sum-marized.The P.ostreatus(PO2)head mushroom and the second tide mushrooms transformed by biological cultivation have high yield,good taste and good economic benefits.The conversion rate is as high as 158%,which has achieved the purpose of high-yield cultivation of P.ostreatus in the laboratory,and at the same time provides an important reference for local farmers in greenhouse cultivation and management techniques.
作者
杨春成
覃雯
武琳
尹立伟
YANG Chuncheng;QINWen;WU Lin;YIN Liwei(The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui,College of Life Science,Anqing Normal University,Anqing 246133,China;Anhui Key Laboratory of Functional Coordination Compounds,School of Chemistry and Chemical Engineering,Anqing Normal University,Anqing 246133,China)
出处
《安庆师范大学学报（自然科学版）》
2022年第3期107-111,122,共6页
Journal of Anqing Normal University(Natural Science Edition)
基金
安徽省教育厅重点项目(KJ2019A0559)
国家自然科学基金(22005004)。
关键词
野生侧耳
糙皮侧耳
分子鉴定
生物栽培
Wild Pleurotus ostreatus
Pleurotus ostreatus
molecular identification
biological cultivation
