摘要
镉(Cd)是对植物毒性最强的重金属之一。克隆Cd转运的关键基因并解析其机理不仅有助于理解植物怎样积累重金属这一基础科学问题,而且可以为低Cd作物的遗传改良提供基因资源。由于高效积累和耐受重金属的特性,超积累植物成为研究离子转运的理想模式植物。本研究从景天科Cd超积累植物伴矿景天(Sedum plumbizincicola)中分离鉴定了SpPCR3基因。它在酵母中表达后能够提高酵母对Cd和铜(Cu)胁迫的抗性。结合其细胞质膜定位的亚细胞定位预测,以及酵母转化子中Cd含量显著减少等结果,我们推测SpPCR3可能通过将Cd外排出细胞从而增加酵母的Cd耐受性。
As a nonessential and toxic metal, cadmium(Cd) generally move into and distribute within plant through transport systems of essential elements. Cloning the key genes of Cd transport and analyzing their mechanism would not only help to understand the basic scientific issues of how plants accumulate Cd, but also provide gene resources for genetic improvement of low-Cd crops. Hyperaccumulator plants have become an ideal model for studying ion transport due to their extreme accumulation and tolerance to heavy metals.Here we isolated a gene named Sp PCR3 from Crassulaceae Cd hyperaccumulator Sedum plumbizincicola. It can increase the tolerance to Cd and copper(Cu) when expressed in yeast. Combining with the prediction of cytoplasmic membrane location and the observation of a significant decrease of Cd content in yeast transformants, we speculate that SpPCR3 may increase the Cd tolerance of yeast by exporting Cd from the cell.
作者
王垚
胡博
邓小秋
蒋佳佳
杜兰芳
彭佳师
WANG Yao;HU Bo;DENG Xiaoqiu;JIANG Jiajia;DU Lanfang;PENG Jjashi(School of Life and Health Sciences,Hunan University of Science and Technology,Xiangtan,Hunan 411201,China;Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization,Hunan University of Science and Technology,Xiangtan,Hunan 411201,China;Key Laboratory of Ecological Remediation and Safe Utlization of Heavy Metal-Plluted Soils,Hunan University of Science and Technology,Xiangtan,Hunan 411201,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2022年第7期1353-1358,共6页
Plant Physiology Journal
基金
国家自然科学基金(U20A2024)
湖南省教育厅资助科研项目(20K055)。
关键词
镉
超积累植物
伴矿景天
转运蛋白
外排
cadmium
hyperaccumulator
Sedum plumbizincicola
transporter
efflux
