摘要
目的分析2015-2018年烟台市人感染戊型肝炎病毒(HEV)基因型别变化,探讨分子流行病特征。方法选取烟台市2015-2018年国家法定传染病报告系统上报的戊型肝炎病例为研究对象,采集血清标本检测HEV-IgM抗体,对血清HEV-IgM阳性病例的血清和粪便标本提取HEV核酸,采用逆转录PCR方法对HEV开放式阅读框架2区域内的核苷酸片段(644 bp)进行扩增,测序后进行系统进化分析。结果共采集360份血清样本,198份(55%)样本HEV-IgM阳性。HEV-IgM阳性的血清标本,HEV核酸检出率27.78%(55/198);粪便标本HEV核酸检测阳性率39.58%(19/48)。总阳性病例数72例,男性48例,女性24例。本次检测的72例HEV病毒株基因型全部是Ⅳ型,以4d亚型为主,占77.78%(56株);其次是4b亚型,占19.44%(14株);4a亚型占2.78%(2株)。72株HEV核苷酸序列的遗传距离为0.002~0.303。56株4d亚型HEV序列与山东地区猪源(KF176351)、羊源(KU904267)、牛源(KU904278)HEV序列的遗传距离为0.021~0.160、0.010~0.178、0.008~0.176。结论基因Ⅳ型HEV在2015-2018年烟台地区占主导趋势,4d亚型为主要流行株。本地人感染HEV可能与进食动物制品或密切接触动物相关。
This study analyzed the genotype changes in hepatitis E virus(HEV)infection in Yantai from 2015 to 2018,to explore the molecular epidemiology characteristics.HEV cases reported to the National Notifiable Disease Reporting Systems from 2015 to 2018 in Yantai were studied.A total of 360 serum samples were collected for HEV-IgM antibody detection.Nucleic acid was extracted from serum and fecal samples of HEV-IgM positive cases.Nested reverse transcription-polymerase chain reaction was used to amplify 644 bp fragments within the HEV open reading frame(ORF2)region,to perform phylogenetic analysis.A total of 198(55%)serum samples were positive for HEV-IgM.The positivity rate of HEV strains was 27.78%(55/198),and that in feces was 39.58%(19/48).The total number of positive HEV strains was 72,in 48 males and 24 females.The genotypes of 72 HEV strains detected in this study were type IV,mainly 4d subtype(77.78%,56 strains),4b subtype(19.44%,14 strains)and 4A subtype(2 strains).The genetic distance of the HEV nucleotide sequence in 72 strains was 0.002-0.303.The genetic distances between 564d HEV subtypes and swine-origin HEV(KF176351),sheep-origin HEV(KU904267)and cattle-origin HEV(KU904278)were 0.021-0.160,0.010-0.178 and 0.008-0.176,respectively.The dominant strain of HEV in Yantai was genotype IV,4d subtype.Animal-borne HEV transmission may be the main source of HEV in this region.
作者
崔伟红
刘娟
宫连凤
颜丙玉
徐迎春
CUI Wei-hong;LIU Juan;GONG Lian-feng;YAN Bing-yu;XU Ying-chun(Shandong College Of Traditional Chinese Medicine,Yantai 264003,China;Yantai Center for Disease Control and Prevention,Yantai 264003,China;Shandong Center for Disease Control and Prevention,Jinan 250012,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2022年第8期703-706,713,共5页
Chinese Journal of Zoonoses
基金
山东省医药卫生科技发展计划项目(No.202012050924)。
