摘要
目的调查烟台市沿海地区人源与猪源戊型肝炎病毒（HEV）基因型别的相关性。方法应用逆转录一巢式聚合酶联反应（RT—nPcR）方法对当地急性散发戊型肝炎患者、正常人群中抗HEV-IgM阳性者和当地养猪场的猪进行HEVRNA检测，并对HEVRNA阳性标本进行克隆测序和序列分析。结果16例急性散发戊型肝炎患者中有7例粪便标本HEVRNA阳性；51份IgM阳性正常人群血清标本中有1份HEVRNA阳性；34份猪胆汁标本中有1份HEVRNA阳性。序列分析发现该地区HEV人株与猪株在ORF2部分区域的核苷酸序列同源性为87％～98．1％。7株患者的戊肝病毒基因型和1株猪的戊肝病毒基因型均为Ⅳ型，基因序列同源性在87％～98．1％之间；其中有6例患者和猪的基因序列同源性在93．9％～98．1％之间，为Ⅳ型a亚型；1例患者和猪的基因序列同源性为87％，为Ⅳ型d亚型。正常人群的1例戊肝病毒基因型为Ⅰ型d亚型。该地区人与猪HEV的ORF2的部分基因片段与HEVⅠ～Ⅳ型的代表株进行比较，核苷酸序列同源性分别是82．5％～100％，81．7％～92．9％，81．4％～93．9％，84．9％～100％。结论该地区人群中流行的HEV存在2个基因型3个亚型，主要以基因Ⅳa型为主，与猪群中流行的HEV基因Ⅳa型同源性较高；HEVI型在人群中散在存在。
Objective Survey of the coastal city of Yantai,from human and swine hepatitis E virus （HEV） genotype correlation. Method Application of reverse transcription nested polymerase chain reaction （RT - nPCR） method for local acute sporadic hepatitis E patients, normal population of HEV-IgM positive and local pig farm pigs were HEV RNA detection. And HEV RNA positive samples for cloning sequencing and sequence analysis. Results In 16 patients with acute sporadic hepatitis E in 7 cases of RNA positive stool specimens of HEV; 51 lgM positive sera of normal people in specimens with 1 HEV RNA positive; 34 pig bile specimens with 1 HEV RNA positive. Sequence analysis revealed the region HEV strains and swine strains in the ORF2 region of nucleotide sequence homology is 87% -98.1%. 7 strains of hepatitis E virus genotype in patients and 1 strains of swine hepatitis E virus genotypes are type IV, gene sequence homology between the 87% -98.1% ; there were 6 patients and porcine gene sequence homology in 93.9% -98. 1% between,for type a subtype; 1 patients and porcine gent sequence homology in 87% ,for the type D subtype. Normal population of 1 cases of hepatitis E virus genotype for I type D subtype. Human and porcine HEV ORF2 gene fragment and HEV part Ⅰ～Ⅳ representative strains were compared,and the nucleotide sequence homology were 82.5% - 100% ,81.7% -92.9% ,81.4% -93.9% ,84.9% - 100%. Conclusion The area population prevalence of HEV in the presence of 2 genotype 3 subtype genes,mainly to IV A,in pigs with popular HEV gene with a high homology; HEV type I in the crowd disperses in the presence of.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2012年第1期31-33,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
肝炎病毒
戊型
基因型
序列分析
模型
动物
Hepatitis E virus
Genotype
Sequence analysis
Models,animal
