摘要
目的探究甲状腺术后切口感染的金黄色葡萄球菌(Staphylococcus aureus bacteremia,SAB)调控Toll样受体2(Toll-like receptor 2,TLR2)/PI3K转导通路对甲状腺癌(thyroid carcinoma,TC)细胞凋亡及自噬的影响。方法随机收集接受甲状腺切除术且术后出现SAB感染的TC患者83例作为感染组,另随机选择同期未出现感染的TC患者83例作为对照组,采用RT-qPCR检测患者外周血中TLR2和LC3B mRNA的表达水平,并通过Pearson相关性检验分析二者的相关性。TC细胞SW1736分为对照组、SAB组和SAB+C29组。以菌胞比为0.1∶1的比例加入SAB,通过50μmol/L的C29抑制TLR2蛋白功能后检测各组细胞增殖、凋亡、自噬以及PI3K磷酸化水平。结果感染组患者外周血中TLR2和LC3B mRNA的水平均显著高于对照组(P<0.05),且TLR2 mRNA和LC3B mRNA水平呈正相关(r=0.482,P<0.05)。SAB组的细胞活力、自噬和PI3K磷酸化水平显著高于对照组,凋亡率显著低于对照组(均P<0.05)。SAB+C29组的细胞活力、自噬和PI3K磷酸化水平显著低于SAB组,凋亡率显著高于SAB组(均P<0.05)。结论甲状腺术后切口感染的SAB通过上调TLR2/PI3K通路而促进TC细胞自噬并抑制其凋亡。
Objective To investigate the effect of Staphylococcus aureus bacteremia(SAB)on thyroid carcinoma(TC)cell apoptosis by regulating TLR2/PI3 K transduction pathway after thyroidectomy incision infection and autophagy.Methods 83 TC patients who underwent thyroidectomy and had SAB infection after surgery were randomly selected as infection group,and 83 TC patients without infection during the same period were randomly selected as control group.RT-qPCR was used to detect TLR2 and LC3 B mRNA in peripheral blood of patients.The expression levels were analyzed by Pearson correlation test.TC cells SW1736 were divided into control group,SAB group and SAB+C29 group.SAB was added at a ratio of 0.1:1 to cell ratio,and 50μmol/L of C29 was used to inhibit the function of TLR2 protein,and then the proliferation,apoptosis,autophagy and PI3 K phosphorylation levels of cells in each group were detected.Results The levels of TLR2 and LC3 B mRNA in the peripheral blood of the infected group were significantly higher than those of the control group(P<0.05),and the levels of TLR2 mRNA and LC3 B mRNA were positively correlated(r=0.482,P<0.05).The cell viability,autophagy and PI3 K phosphorylation levels in the SAB group were significantly higher than those in the control group,and the apoptosis rate was significantly lower than that in the control group(all P<0.05).The cell viability,autophagy and PI3 K phosphorylation levels in the SAB+C29 group were significantly lower than those in the SAB group,and the apoptosis rate was significantly higher than that in the SAB group(P<0.05).Conclusion After thyroidectomy,incision-infected SAB promotes autophagy and inhibits apoptosis of TC cells by up-regulating the TLR2/PI3 K pathway.
作者
吴渊
严宏
WU Yuan;YAN Hong(Department of Neck Thyroid and Breast Surgery,People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350001,China)
出处
《中国病原生物学杂志》
CSCD
北大核心
2022年第7期827-830,共4页
Journal of Pathogen Biology
