摘要
目的:探讨反复不明原因自然流产患者血清、绒毛和蜕膜中miR-129-5p表达水平及临床意义.方法:选择2018年10月-2021年9月本院收治的148例反复不明原因自然流产患者为流产组,正常早期妊娠人工流产者80例为对照组,检测两组血清、绒毛和蜕膜中miR-129-5p表达水平并分析与血清孕酮(P)、人绒毛膜促性腺激素(hCG)、白介素2(IL-2)、血管内皮生长因子(VEGF)水平的相关性,及对反复不明原因自然流产患者的诊断价值.结果:流产组血清(0.33±0.04)、绒毛(0.30±0.03)和蜕膜(0.28±0.02)miR-129-5p表达水平均低于对照组(1.12±0.11、1.35±0.13、1.13±0.12),血清P、hCG、VEGF水平均低于对照组,IL-2水平高于对照组(均P<0.05).Pearson相关分析显示,流产组血清、绒毛和蜕膜miR-129-5p表达水平与血清P、hCG、VEGF均呈正相关,与IL-2水平呈负相关(均P<0.05).受试者工作特征曲线分析显示,血清、绒毛、蜕膜miR-129-5p诊断反复不明原因自然流产的曲线下面积分别为0.820、0.912、0.856,截断值分别为0.60、0.83、0.72,敏感度分别为74.8%、81.0%、71.6%,特异度分别为81.2%、89.6%、91.7%.结论:反复不明原因自然流产患者血清、绒毛和蜕膜中miR-129-5p表达水平均异常降低,且与炎症反应、抑制血管生成指标相关,对诊断反复不明原因自然流产有一定临床价值.
Objective: To investigate the expression levels of miR-129-5 p in serum, villi, and decidua of patients with recurrent unexplained spontaneous abortion(RUSA), and to study its clinical significance. Methods: A total of 148 patients with RUSA were selected in research group from October 2018 to September 2021, and 80 normal pregnant women who wanted early artificial abortion were selected in control group during the same period. The levels of miR-129-5 p in the serum, villi, and decidua of the women in the two groups were detected. The correlation between the levels of miR-129-5 p in serum, chorion, and decidua of the women and the levels of their serum progesterone(P), human chorionic gonadotropin(hCG), interleukin 2(IL-2), and vascular endothelial growth factor(VEGF). The value of miR-129-5 p expression in serum, villi, and decidua of the women for diagnosing their RUSA was analyzed. Results: The levels of miR-129-5 p in serum(0.33±0.04), in villi(0.30±0.03), and in decidua(0.28±0.02) of the women in the research group were significantly lower than those(1.12±0.11, 1.35±0.13, and 1.13±0.12) of the women in the control group. The levels of serum P, hCG, and VEGF of the women in the research group were significantly lower than those of the women in the control group, and the serum IL-2 level of the women in the research group was significantly higher than that of the women in the control group(all P<0.05). Pearson correlation analysis showed that the level of miR-129-5 P in serum, in villi, or in decidua of the women in the research group was positively correlated with their levels of serum P, hCG and VEGF, but was negatively correlated with their IL-2 level(all P<0.05). The receiver operating characteristic(ROC)curve analysis showed that the area under the curve of the level of miR-129-5Pin serum,in villi,or in decidua of the women for the diagnosing their RUSA was 0.820,0.912,or 0.856,the cut-off value of which was 0.60,0.83,or 0.72,the sensitivity of which was 74.8%,81.0%,or 71.6%,respectively,and the specificity of which was 81.2%,89.6%,or 91.7%,respectively.Conclusion:The expressions of miR-129-5p in the serum,villi,and decidua of the women with RUSA are abnormally decreased and are closely related to the inflammatory response and inhibiting angiogenesis of the women,which have some values for diagnosing RUSA of the women.
作者
李莉
王媛
石芳
LI Li;WANG Yuan;SHI Fang(Yan'an Hospital of Traditional Chinese Medicine,Shaanxi Province,716000;YAU Affiliated Hospital of Yan'an University)
出处
《中国计划生育学杂志》
2022年第7期1510-1513,共4页
Chinese Journal of Family Planning
