摘要
【目的】探讨Slit2、Robol蛋白在胰腺癌组织中的表达及其与微血管密度(MVD)关系。【方法】免疫组化SP法检测30例胰腺癌组织及30例癌旁组织中Slit2、Robol蛋白的表达水平,比较不同临床病理学行为下Slit2蛋白和Robol蛋白的表达变化,分析Slit2、Robol蛋白的表达水平与MVD关系。【结果】在胰腺癌组织中Slit2、Robol蛋白阳性表达率分别为60.00%和70.00%,显著癌旁组织中的33.33%和40.00%,其差异具有统计学意义(P<0.05);且Slit2与Robol蛋白阳性表达呈正相关(r=0.818,P<0.05)。胰腺癌组织MVD表达高于癌旁组织,其差异具有统计学意义(t=9.475,P<0.05);Slit2.Robol阳性表达的胰腺癌组织MVD明显大于表达阴性的胰腺癌组织(t=2.048,P<0.05,t=2.897,P<0.05);Slit2、Robol蛋白表达及MVD与胰腺癌大小、病理类型、临床分期、淋巴结转務相关(P<0.05)。【结论】Slit2、Robol蛋白在胰腺癌组织中表达增高,与胰腺癌的生长、侵袭及转移密切相关,其机制可能是Slit2/Robol信号通路通过诱导肿瘤血管形成促进了胰腺癌的发生发展。
【Objective】To investigate the expression of Slit2 and Robol proteins in pancreatic cancer and their relationship with microvessel density(MVD).【Methods】Immunohistochemical staining SP method was used to detect the expression of Slit2 and Robol proteins in 30 cases of pancreatic cancer tissues and 30 cases of adjacent tissues.The expression changes of Slit2 protein and Robol protein under different clinicopathological conditions were observed.The microvessel density(MVD)marker CD105 was detected to study the relationship between Slit2/Robol protein expression and MVD in pancreatic cancer.【Results】The positive rates of Slit2 and Robol were 60.00%and 70.00%in pancreatic cancer tissues,respectively;and the positive rates in the adjacent tissues were 33.33% and 40.00%,respectively.The differences between the two types of tissues were statistically significant(P<0.05).There was a positive correlation between the positive expression of Slit2 protein and Robol protein(r=0.818,P<0.05).The expression of MVD in pancreatic cancer tissues was higher than that in the adjacent tissues(t=9.475,P<0.05).The MVD in pancreatic cancer tissues with positive expression of Slit2 and Robol was much higher than that in pancreatic cancer tissues with negative expression of Slit2 and Robot 1(t=2.048,P<0.05,t=2.897,P<0.05).Expression of Slit2,Robol and MVD were significantly associated with tumor size,pathological type,clinical stage,and lymph node metastasis of pancreatic cancer(P<0.05).【Conclusion】The increased expression of Slit2 protein and Robol protein in pancreatic cancer tissue is closely related to the growth,invasion and metastasis of pancreatic cancer.The mechanism may be that Slit2/Robol signaling pathway promotes the occurrence and development of pancreatic cancer by inducing tumor angiogenesis.The expression intensity of Slit2/Robol and the enumerations of MVD can be used to predict the progression and prognosis of pancreatic cancer.
作者
梁刚
王智翔
何盟国
杨学超
LIANG Gang;WANG Zhi-xiang;HE Meng-guo(Department of Hepatobiliary Surgery,Nuclear Industry 215 Hospital of Shaanxi,Xianyang 712000,China)
出处
《医学临床研究》
CAS
2020年第1期95-97,101,共4页
Journal of Clinical Research
